The flowers extracts of Citrus aurantium regulates fat metabolism in obese C57BL/6J mice by improving intestinal microbiota disorders

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-02-13 DOI:10.1002/jsfa.14139

Ke-Xin Hao, Chun-Yan Shen, Jian-Guo Jiang

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引用次数: 0

Abstract

Background

Obesity can lead to many diseases such as diabetes, hypertension, cancer and cardiovascular diseases, which seriously affect people's quality of life and health.

Aims of the study

To investigate the main components and potential of n-butanol extract from Citrus aurantium L. var. amara Engl to reduce lipid accumulation and to explore its modulatory effects on the gut microbiota.

Methods

The main components of n-butanol extract were analyzed using liquid chromatography quadrupole trap mass spectrometry (LC-QTRAP-MS) and a high-fat diet-induced obese mouse model was established to analyze its effects on the determination of gene expression levels and intestinal microbiota using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and 16S rRNA gene sequence method.

Results

The n-butanol extract mainly consists of 15 components, and it could significantly inhibit weight gain, reduce liver coefficient and improve oxidative damage. By regulating the expression of related genes, it inhibited hepatic steatosis and hypertrophy of epididymal tissue. The n-butanol extract increased the diversity of intestinal microbiota, improved the composition and structure of the flora, and reversed the high-fat diet-induced disturbance of intestinal microbiota in mice.

Conclusion

These results indicated that the n-butanol extract of C. aurantium could inhibit lipid accumulation and provide a more comprehensive basis for the development and utilization of C. aurantium in anti-obesity activity. © 2025 Society of Chemical Industry.

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柑橘花提取物通过改善肠道菌群紊乱调节肥胖C57BL/6J小鼠的脂肪代谢。

背景：肥胖可导致糖尿病、高血压、癌症、心血管疾病等多种疾病，严重影响人们的生活质量和健康。研究目的：研究金柑橘正丁醇提取物的主要成分及其降低脂质积累的作用，探讨其对肠道微生物群的调节作用。方法：采用液相色谱-四极杆陷阱质谱法（LC-QTRAP-MS）对正丁醇提取物的主要成分进行分析，建立高脂饮食致肥胖小鼠模型，采用反转录定量聚合酶链反应（RT-qPCR）和16S rRNA基因序列法分析正丁醇提取物对基因表达水平和肠道菌群测定的影响。结果：正丁醇提取物主要由15种成分组成，具有显著抑制体重增加、降低肝脏系数、改善氧化损伤的作用。通过调节相关基因的表达，抑制肝脂肪变性和附睾组织肥大。正丁醇提取物增加了小鼠肠道菌群的多样性，改善了肠道菌群的组成和结构，逆转了高脂肪饮食引起的肠道菌群紊乱。结论：上述结果提示金缕兰正丁醇提取物具有抑制脂肪堆积的作用，为金缕兰抗肥胖活性的开发利用提供了更全面的依据。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.