The extracytoplasmic sigma factor σ^X supports biofilm formation and increases biocontrol efficacy in Bacillus velezensis 118.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-02-13 DOI:10.1038/s41598-025-89284-7

Yanfei Cai, Huan Tao, Ahmed Gaballa, Hualiang Pi, John D Helmann

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Abstract

Plant growth promoting rhizobacteria (PGPR) offer an environmentally friendly and sustainable approach to combat pathogens and enhance crop production. The biocontrol activity of PGPR depends on their ability to colonize plant roots and synthesize antimicrobial compounds that inhibit pathogens. However, the regulatory mechanisms underlying these processes remain unclear. In this study, we isolated and characterized Bacillus velezensis 118, a soil isolate that exhibits potent biocontrol activity against Fusarium wilt of banana. Deletion of sigX, encoding an extracytoplasmic function (ECF) sigma factor previously implicated in controlling biofilm architecture in B. subtilis, reduced biocontrol efficacy. The B. velezensis 118 sigX mutant displayed reduced biofilm formation but had only a minor defect in swarming motility and a negligible impact on lipopeptide production. These findings highlight the importance of regulatory processes important for root colonization in the effectiveness of Bacillus spp. as biocontrol agents against phytopathogens.

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胞质外sigma因子σX支持芽孢杆菌118的生物膜形成，提高了芽孢杆菌118的生物防治效果。

促进植物生长的根瘤菌（PGPR）提供了一种环境友好和可持续的方法来对抗病原体和提高作物产量。PGPR的生物防治活性取决于它们定殖植物根系和合成抑制病原体的抗菌化合物的能力。然而，这些过程背后的调控机制仍不清楚。在这项研究中，我们分离并鉴定了一种对香蕉枯萎病具有有效生物防治活性的土壤分离物velezensis 118。sigX编码的胞质外功能（ECF） sigma因子先前涉及控制枯草芽孢杆菌的生物膜结构，其缺失降低了生物防治效果。velezensis 118 sigX突变体显示生物膜形成减少，但在群体运动方面只有轻微缺陷，对脂肽产生的影响可以忽略。这些发现强调了调控过程在芽孢杆菌作为植物病原体生物防治剂的有效性中对根定植的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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