Laser Ablation Coupled with LC-ICP-MS for Local Speciation of Trace Elements in Tissues

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-02-13 DOI:10.1021/acs.analchem.5c00153

Makiko Iwase, Yu-Ki Tanaka, Yasunori Fukumoto, Noriyuki Suzuki, Yasumitsu Ogra

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引用次数: 0

Abstract

The body mitigates the toxic effects of metals through diverse detoxification mechanisms that are activated depending on the chemical species and the burden of metals in each tissue. In this regard, analytical methods that can obtain information on the chemical form and the abundance of metals are required to elucidate the full range of detoxification mechanisms. Laser ablation (LA) is used to trim a specific microregion from tissue sections and visualize elements in it. Speciation analysis by liquid chromatography (LC) hyphenated to inductively coupled plasma mass spectrometry (ICP-MS) has been optimized for microvolume samples from small tissue sections. In this study, we developed a combined method of LA and LC-ICP-MS (LA/LC-ICP-MS) and applied it to rat brain and kidney tissues. Differences in copper (Cu) and zinc (Zn) abundance in each brain tissue region are reflected in the peak intensities of metallothioneins (MTs) detected by LC-ICP-MS analysis. In addition to revealing differences in the distribution and the concentration of mercury (Hg) in the kidneys of rats exposed to inorganic mercury (iHg) or methylmercury (MeHg) by LA-ICP-MS, we also revealed differences in the type of proteins that bind these Hg species by LC-ICP-MS. We found that in the iHg-exposed group, MT induction occurred mainly in the renal cortex and the outer medulla with elevated Cu and Zn, whereas in the MeHg-exposed group, Hg was mainly bound to hemoglobin (Hb). LA/LC-ICP-MS can simultaneously provide qualitative and quantitative information on metals in a small tissue region.

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.