{"title":"pacsin1 inhibits antiviral immunity by promoting MITA degradation through autophagy in miiuy croaker, Miichthys miiuy.","authors":"Wenxin Li, Wenjing Dong, Zhihuang Zhu, Baolan Cao, Tianjun Xu, Yuena Sun","doi":"10.1016/j.fsi.2025.110182","DOIUrl":null,"url":null,"abstract":"<p><p>Pacsin1 is a crucial protein involved in vesicle formation and transport, and its role in neuronal development and cytosolic dynamics has been extensively studied. However, its involvement in immune regulation still needs to be better understood. In this study, we show that pacsin1 exerts a negative regulatory effect on RLR-mediated signaling pathways activated by SCRV or poly(I:C), thereby inhibiting MITA-mediated antiviral responses. Mechanistically, pacsin1 facilitates the degradation of MITA, thus impeding immune signaling. Additionally, overexpression of pacsin1 promotes the conversion of LC3B-I to LC3B-II, while treatment with the autophagy inhibitor ammonium chloride results in the accumulation of LC3B-II and prevents pacsin1-mediated MITA degradation. Our findings suggest that pacsin1 targets MITA for autophagic degradation, thereby suppressing the innate antiviral response in fish.</p>","PeriodicalId":12127,"journal":{"name":"Fish & shellfish immunology","volume":" ","pages":"110182"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish & shellfish immunology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.fsi.2025.110182","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Pacsin1 is a crucial protein involved in vesicle formation and transport, and its role in neuronal development and cytosolic dynamics has been extensively studied. However, its involvement in immune regulation still needs to be better understood. In this study, we show that pacsin1 exerts a negative regulatory effect on RLR-mediated signaling pathways activated by SCRV or poly(I:C), thereby inhibiting MITA-mediated antiviral responses. Mechanistically, pacsin1 facilitates the degradation of MITA, thus impeding immune signaling. Additionally, overexpression of pacsin1 promotes the conversion of LC3B-I to LC3B-II, while treatment with the autophagy inhibitor ammonium chloride results in the accumulation of LC3B-II and prevents pacsin1-mediated MITA degradation. Our findings suggest that pacsin1 targets MITA for autophagic degradation, thereby suppressing the innate antiviral response in fish.
期刊介绍：
Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.
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