In Silico Analysis and Characterization of the Immunogenicity of Toxoplasma gondii Rhoptry Protein 18.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Bioinformatics and Biology Insights Pub Date : 2025-02-07 eCollection Date: 2025-01-01 DOI:10.1177/11779322251315924
Masoud Foroutan, Amir Karimipour-Saryazdi, Ali Dalir Ghaffari, Hamidreza Majidiani, Arezo Arzani Birgani, Elaheh Karimzadeh-Soureshjani, Shahrzad Soltani, Hany M Elsheikha
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引用次数: 0

Abstract

Rhoptry protein 18 (ROP18) is a key virulence factor secreted into host cells during the invasion of Toxoplasma gondii (T. gondii) and plays an important role in the pathogenesis of infection. Due to its potential as a vaccine candidate, this study aimed to characterize several properties of the T. gondii ROP18 (TgROP18) protein to support its inclusion in vaccine formulations. Using a range of bioinformatics tools, we investigated its T-cell and B-cell epitopes, physicochemical properties, subcellular localization, transmembrane domains, and tertiary and secondary structures. Our analysis revealed 48 post-translational modification sites in TgROP18. The secondary structure was composed of 4.87% beta-turns, 38.45% random coils, 42.42% alpha helices, and 14.26% extended strands. Several potential T- and B-cell epitopes were identified on ROP18. The Ramachandran plot of both crude and refined models showed that 85.8% and 95.3% of the amino acid residues, respectively, fell within favored regions, indicating energetically stable conformations. Allergenicity and antigenicity assessments indicated that TgROP18 is a nonallergenic, immunogenic protein. Predictions using the C-ImmSim server suggest that TgROP18 can stimulate humoral and cell-mediated immune responses, based on antibody titers and cytokine profiles following antigen administration. These findings provide baseline data for future investigations focused on the potential of TgROP18 in developing therapeutic strategies against toxoplasmosis.

刚地弓形虫虫体蛋白18免疫原性的计算机分析与表征。
弓形虫蛋白18 (ROP18)是弓形虫入侵宿主细胞时分泌到宿主细胞中的关键毒力因子,在感染的发病机制中起重要作用。由于其作为候选疫苗的潜力,本研究旨在表征弓形虫ROP18 (TgROP18)蛋白的几种特性,以支持将其纳入疫苗配方。利用一系列生物信息学工具,我们研究了它的t细胞和b细胞表位、物理化学性质、亚细胞定位、跨膜结构域和三级和二级结构。我们的分析发现了TgROP18的48个翻译后修饰位点。二级结构由4.87%的β匝、38.45%的随机线圈、42.42%的α螺旋和14.26%的延伸链组成。在ROP18上发现了几个潜在的T细胞和b细胞表位。粗糙模型和精细模型的Ramachandran图分别显示85.8%和95.3%的氨基酸残基落在有利区域内,表明能量稳定的构象。致敏性和抗原性评估表明,TgROP18是一种非致敏性、免疫原性的蛋白。使用C-ImmSim服务器的预测表明,基于抗原给药后的抗体滴度和细胞因子谱,TgROP18可以刺激体液和细胞介导的免疫反应。这些发现为未来研究TgROP18在开发弓形虫病治疗策略方面的潜力提供了基线数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioinformatics and Biology Insights
Bioinformatics and Biology Insights BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.80
自引率
1.70%
发文量
36
审稿时长
8 weeks
期刊介绍: Bioinformatics and Biology Insights is an open access, peer-reviewed journal that considers articles on bioinformatics methods and their applications which must pertain to biological insights. All papers should be easily amenable to biologists and as such help bridge the gap between theories and applications.
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