{"title":"Characterization and genomic analysis of Cronobacter sakazakii phage JC03, a new member of the genus Pseudotevenvirus","authors":"Jie Jiang, Jingying Sai, Xin Huang, Lingling Sun, Jinghua Li, Honglan Huang, Yanbo Sun, Chunyan Zhao","doi":"10.1007/s00705-025-06245-1","DOIUrl":null,"url":null,"abstract":"<div><p>The <i>Cronobacter sakazakii</i>-infecting phage JC03 was isolated and characterized. Its genome is composed of 177,089 bp of double-stranded DNA, contains 268 predicted open reading frames (ORFs) and two tRNA genes, and it has a G + C content of 44.67%. The predicted ORFs were classified into functional groups, including DNA replication/modification, assembly/packaging, phage structure, host lysis, and hypothetical proteins, and no genes associated with antibiotic resistance or virulence were found. Based on the results of gene annotation, genome features, and comparative genomics, we propose that JC03 is a new member of the genus <i>Pseudotevenvirus.</i></p></div>","PeriodicalId":8359,"journal":{"name":"Archives of Virology","volume":"170 3","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Virology","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s00705-025-06245-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VIROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The Cronobacter sakazakii-infecting phage JC03 was isolated and characterized. Its genome is composed of 177,089 bp of double-stranded DNA, contains 268 predicted open reading frames (ORFs) and two tRNA genes, and it has a G + C content of 44.67%. The predicted ORFs were classified into functional groups, including DNA replication/modification, assembly/packaging, phage structure, host lysis, and hypothetical proteins, and no genes associated with antibiotic resistance or virulence were found. Based on the results of gene annotation, genome features, and comparative genomics, we propose that JC03 is a new member of the genus Pseudotevenvirus.
期刊介绍:
Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.