Molecular characterization of a novel deltaflexivirus from the plant-pathogenic fungus Neopestalotiopsis nebuloides strain N-7

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-02-08 DOI:10.1007/s00705-025-06219-3

Caiming Wu, Siyu Zhou, Changping Xie, Daipeng Chen, Li Zheng

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Abstract

The full genome sequence of a positive-sense (+) single-stranded (ss) RNA virus, which we have named "Neopestalotiopsis nebuloides deltaflexivirus 1" (NnDFV1), from Neopestalotiopsis nebuloides strain N-7 was sequenced and analyzed. The NnDFV1 genome is 7,719 nucleotides in length with a GC content of 49%, excluding the poly(A) tail, and contains a large open reading frame (ORF1) and three smaller ORFs (2–4). ORF1 encodes a replication-associated polyprotein (RP) consisting of three conserved domains: viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRp), whereas ORFs 2–4 encode three hypothetical proteins (18–20 kDa). Phylogenetic analysis showed that NnDFV1 formed a distinct clade together with Pestalotiopsis deltaflexivirus 1 (PDFV1), which is a new member of the genus Deltaflexivirus within the family Deltaflexiviridae. This is the first report of a novel deltaflexivirus found in the phytopathogenic fungus Neopestalotiopsis nebuloides.

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.