Universal, Sensitive, and Visual Sandwich-type Biosensor Based on Nanogold-catalyzed Reduction and its Application for Detecting C-Reactive Protein in Serum by a Portable Colorimeter

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-02-07 DOI:10.1016/j.snb.2025.137408

Runhui Zhang, Huaze Shao, Xiumei Hu, Yongshi Liang, Hanren Chen, Shiquan Zheng, Lihong Liu

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引用次数: 0

Abstract

We proposed a low-cost, high sensitive and universal point-of-care testing method of different dimension biomarkers based on the target-mediated catalytic reduction suppression of double-sequence aptamers-modified gold nanoparticles (dsApts@AuNPs) and NaBH₄ on the organic dyes (T-AAND). Herein, carcinoembryonic antigen, alpha-fetoprotein, C-reactive protein (CRP), thrombin, lipopolysaccharide, Staphylococcus aureus, and Escherichia coli were chosen as model biomarkers and all successfully detected, with limit of detections of 6.5 pg/mL, 12.7 pg/mL, 79.7 pg/mL, 34.2 fM, 459 fM, 718 CFU/mL, and 830 CFU/mL, respectively. Remarkably, the assay is completed in a single EP tube within 40 minutes, with on-site quantification performed using a $70 portable colorimeter and the cost of reagents for each test is $0.75. The CRP levels of 31 patients and 11 healthy volunteers achieved with the latex-enhanced immunoturbidimetry were high consistency with those of the spectrophotometry (r = 0.9964) and the colorimeter (r = 0.9619), demonstrating the accuracy of the new method. In conclusion, the T-AAND integrated a colorimeter is a superior approach for detecting biomarkers, especially in resource-limited settings.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.