Casein Glycomacropeptide Regulates Gene Expression in Intestinal Epithelial Cells: Effect of Simulated Gastrointestinal Digestion and Peptide Microencapsulation

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-02-08 DOI:10.1021/acs.jafc.4c10146

Raúl E. Cian, Mireia Tena-Garitaonaindia, Fermín Sánchez de Medina, Olga Martínez-Augustin

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引用次数: 0

Abstract

κ-Casein glycomacropeptide (GMP) exerts anti-inflammatory and immune modulatory effects. A bovine GMP concentrate and its in vitro digestion product were obtained. GMP was also microencapsulated with phycocolloids and further digested. These products were tested in three-dimensional (3D) and open monolayer two-dimensional (2D) mouse jejunal organoids. Almost no effect was observed on the 2D organoids. In 3D organoids, GMP induced intestinal proliferation (Axin2, Pcna) and differentiation (Vil1, Alpl) genes together with Muc3, antibacterial genes (Lyz1, Pla2g2a), and Cxcl1. GMP also induced interferon I defense genes (Ifnb1, Ifr3, Oas2, Oas3, Rnasel) under basal conditions and in TNF-stimulated organoids. In vitro digestion abrogated the effects of GMP and induced new genes (Lgr5, Olfm4, and Lct). In TNF-stimulated organoids, digested GMP repressed multiple genes. Encapsulation largely preserved the GMP effects. In conclusion, GMP showed differential effects in 3D and 2D organoids. The effects of digestion peptides were also different, suggesting distinct potential as functional foods.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.