Complementation with TaNCL2-A reinstates growth and abiotic stress response in atncl mutant of Arabidopsis

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-02-04 DOI:10.1016/j.plantsci.2025.112411

Ishu, Shumayla, Madhu, Santosh Kumar Upadhyay

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引用次数: 0

Abstract

The sodium/calcium exchanger-like (NCL) transporters are members of Ca^2 +/Cation antiporters (CaCAs) family, localized at the tonoplast, and primarily involved in Ca²⁺ homeostasis and stress response. They transport Ca²⁺ to the cytosol and sequester cytosolic Na⁺ into the vacuole. Therefore, the atncl mutant of Arabidopsis thaliana is prone to salinity stress. The functional complementation of TaNCL2-A of Triticum aestivum improved abiotic stress response and various morpho-physio-biochemical parameters in atncl mutant. The TaNCL2-A complementation increased the seed germination rate and root length of atncl mutant during salinity and drought stress conditions. The exogenous Ca²⁺ application further improved the stress tolerance in the complemented lines. The results suggested that the modulation of cytosolic Ca²⁺ by TaNCL2-A expression and/or exogenous Ca²⁺ application could reinstate growth and abiotic stress response in atncl mutant. TaNCL2-A also reduced the impact of ABA on seed germination. In addition, exogenous IAA induced lateral roots formation in all the lines. Biochemical and physiological analyses revealed increased proline, chlorophylls, carotenoids and relative water content (RWC), and reduced malondialdehyde (MDA), H₂O₂ and relative electrical conductivity (REC) in TaNCL2-A complemented lines. The results highlighted the function of TaNCL2-A gene in stress response, and its potential application in crop improvement strategies in future studies.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.