Prevalence of Epstein-Barr Virus, Cytomegalovirus, and Periodontopathic Bacteria in Patients With Periodontitis: A Case–Control Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2025-02-06 DOI:10.1002/cre2.70084

Alicia Herrero-Sánchez, Evelina Haroyan-Darbinyan

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引用次数: 0

Abstract

Objectives

Opportunistic viruses such as cytomegalovirus (CMV) and Epstein-Barr virus (EBV) have been detected in gingival crevicular fluid (GCF) and saliva of patients with periodontal disease. However, the relationship between herpesviruses and periodontitis remains obscure. The aim of this case–control study was the detection and association of CMV and EBV with periodontitis.

Material and Methods

Forty-eight adults were included in this study: 24 patients with periodontitis (CP) and 24 periodontally healthy individuals (HS). All patients underwent periodontal examination, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), and bleeding on probing (BOP). Subgingival biofilm samples were collected from all patients and real-time PCR was performed for viral and bacterial detection. The odds ratio (OR) was calculated, and the chi-squared test or Fisher's exact test was performed to analyze the significant differences.

Results

EBV was detected only in one healthy patient meanwhile no CMV was found. No statistically significant differences were found between the periodontal clinical parameters of EBV-positive patients and the negative ones: PI (p = 0.090), PD (p = 0.857), CAL (p = 0.801), and BOP (p = 0.104). Except for Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Prevotella intermedia (Pi), and Treponema denticola (Td) showed a statistically significant association (p < 0.001) with the clinical periodontal parameters. Aa presence was not statistically associated with periodontal sites (p < 0.296). Tf and Pg were the most frequently detected periodontopathic bacteria in the CP group (91.7% sites).

Conclusion

The present case–control study showed that the prevalence of EBV and CMV did not show significant differences between the groups evaluated in the Spanish population.

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Epstein-Barr病毒、巨细胞病毒和牙周炎细菌在牙周炎患者中的流行：一项病例-对照研究

目的在牙周病患者龈沟液（GCF）和唾液中检测巨细胞病毒（CMV）和eb病毒（EBV）等机会病毒。然而，疱疹病毒与牙周炎之间的关系仍不清楚。本病例对照研究的目的是检测巨细胞病毒和EBV与牙周炎的关系。材料与方法48例成人：24例牙周炎患者（CP）和24例牙周健康者（HS）。所有患者均接受牙周检查，包括探诊深度（PD）、临床附着损失（CAL）、菌斑指数（PI）和探诊出血（BOP）。采集所有患者的龈下生物膜标本，采用实时荧光定量PCR检测病毒和细菌。计算比值比（OR），采用卡方检验或Fisher精确检验分析差异的显著性。结果1例健康患者仅检出EBV，未检出CMV。ebv阳性患者与阴性患者牙周临床参数PI （p = 0.090）、PD （p = 0.857）、CAL （p = 0.801）、BOP （p = 0.104）差异无统计学意义。除放线菌聚集菌（Aa）外，牙龈卟啉单胞菌（Pg）、连翘Tannerella （Tf）、中间普雷伏菌（Pi）和牙密螺旋体（Td）与临床牙周参数的相关性均有统计学意义（p < 0.001）。Aa的存在与牙周部位无统计学相关性（p < 0.296）。在CP组中，Tf和Pg是最常见的牙周病细菌（91.7%）。结论：本病例对照研究显示，在西班牙人群中，EBV和CMV的患病率在各组间没有显着差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.