Preparation of nanocube PtRhIr nanozyme and its application in ultrasensitive lateral flow immunoassay

IF 2.7 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials Letters Pub Date : 2025-02-02 DOI:10.1016/j.matlet.2025.138123

Ying Zhang , Bin Liu , Haibo Zhang

引用次数: 0

Abstract

The sensitivity of lateral flow immunoassay (LFIA) should be enhanced for detecting low-abundance markers. This study introduces a one-step synthesis method for a cuboidal nanozyme containing Pt, Rh, and Ir (PtRhIr) with a rough surface. The PtRhIr nanozyme exhibited peroxidase-like activity and effectively catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate a blue product. Enzyme kinetics revealed that the PtRhIr nanozyme has an affinity for the H₂O₂ substrate (6.55 mM), which is comparable to that of horseradish peroxidase. When the PtRhIr-nanozyme-incorporated LFIA was used for detecting the model marker hCG, the sensitivity increased by 7.8-fold compared to that achieved using a conventional Au nanoparticles based LFIA, achieving a visible detection limit of 3.2 IU/L of hCG.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Materials Letters 工程技术-材料科学：综合

CiteScore

5.60

自引率

3.30%

发文量

1948

审稿时长

50 days

期刊介绍： Materials Letters has an open access mirror journal Materials Letters: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Materials Letters is dedicated to publishing novel, cutting edge reports of broad interest to the materials community. The journal provides a forum for materials scientists and engineers, physicists, and chemists to rapidly communicate on the most important topics in the field of materials. Contributions include, but are not limited to, a variety of topics such as: • Materials - Metals and alloys, amorphous solids, ceramics, composites, polymers, semiconductors • Applications - Structural, opto-electronic, magnetic, medical, MEMS, sensors, smart • Characterization - Analytical, microscopy, scanning probes, nanoscopic, optical, electrical, magnetic, acoustic, spectroscopic, diffraction • Novel Materials - Micro and nanostructures (nanowires, nanotubes, nanoparticles), nanocomposites, thin films, superlattices, quantum dots. • Processing - Crystal growth, thin film processing, sol-gel processing, mechanical processing, assembly, nanocrystalline processing. • Properties - Mechanical, magnetic, optical, electrical, ferroelectric, thermal, interfacial, transport, thermodynamic • Synthesis - Quenching, solid state, solidification, solution synthesis, vapor deposition, high pressure, explosive