GmERF57 negatively regulates root development and phosphate absorption in soybean

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-02-05 DOI:10.1016/j.stress.2025.100763

Hongqing Zhu , Dandan Hu , Yifei Yang , Xuhao Zhai , Shanshan Zhang , Mengshi He , Huifang Zuo , Lina Zhang , Mengjun Xu , Shanshan Chu , Haiyan Lü , Hengyou Zhang , Yu Zhang , Dan Zhang

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引用次数: 0

Abstract

Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilization and uptake efficiency in plants is crucial for sustainable agriculture. Previously, we identified soybean Ethylene Response Factor 57 (GmERF57) as a candidate gene responsible for q20, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). Population genomics analysis revealed that GmERF57 has undergone artificial selection, and allelic distribution analysis significantly correlated with traits associated with LP tolerance in soybean. Haplotype 2 (Hap2), carrying the T to C single nucleotide polymorphism (SNP), represents the optimal allele favoring LP tolerance. Silencing GmERF57 expression promoted root development and enhanced plant Pi uptake; conversely, overexpression of GmERF57 yielded contrasting phenotypes compared to silenced roots. We further uncovered that GmERF57 physically interacts with GmTUB1 (β-tubulin protein) and modulates soybean root architecture and Pi uptake capacity by downregulating GmTUB1 and other Pi starvation response genes. Overall, the results identified GmERF57 as a QTL gene associated with LP tolerance, elucidated its role in regulating LP tolerance, and further identified an optimal haplotype to facilitate breeding of LP-tolerant soybean cultivars.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.