VEGFR3 pathway in corneal transplantation.

IF 3 3区 医学 Q1 OPHTHALMOLOGY
Mariateresa Laborante, Alessandra Micera, Daniele Gaudenzi, Andrea De Luca, Chiara De Gregorio, Francesco Cutrupi, Graziana Esposito, Bijorn Omar Balzamino, Antonio Laborante, Marco Coassin, Antonio Di Zazzo
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Abstract

Purpose: Corneal transplantation, a common procedure in ophthalmology, faces challenges in high-risk (HR) cases due to inflammation and vascularization of the host bed, leading to graft rejection. Despite advancements in surgical techniques and therapeutics, preventing rejection in HR cases remains elusive. This study investigates the role of the vascular endothelial-derived growth factor (VEGF)-C/D-VEGFR3 pathway in corneal transplantation, focusing on its impact on inflammation and immune response.

Methods: In this study, 42 eyes of 42 patients were evaluated, 24 of whom underwent corneal transplantation, with follow-up visits at 90, 180 and 360 days post-transplantation. Clinical assessments included visual acuity, corneal oedema, endothelial cell count and vascularization. Molecular analyses were performed to measure VEGFR3, VEGF-C, VEGF-D, VEGF-A and inflammatory markers.

Results: Results revealed a distinct pattern of VEGF-C/D and VEGFR3 expression in HR versus low-risk (LR) transplants, correlating with inflammatory markers and clinical outcomes. In HR cases, elevated VEGFR3 expression was associated with increased inflammatory markers (Intercellular Adhesion Molecule 1 (ICAM-1) and Human Leukocyte Antigen - DR isotype (HLA-DR)) and graft rejection risk.

Conclusion: These findings underscore the importance of understanding the VEGF-C/D-VEGFR3 pathway in modulating immune responses and inflammation in corneal transplantation, particularly in HR cases. Targeting this pathway could offer novel therapeutic avenues to mitigate inflammation and improve graft survival. Further research is warranted to elucidate the underlying mechanisms and validate these findings, potentially enhancing transplant outcomes, especially in HR patients.

角膜移植中的VEGFR3通路。
目的:角膜移植是眼科常见的手术,但由于宿主床的炎症和血管化,导致移植排斥反应,在高危病例中面临挑战。尽管手术技术和治疗方法取得了进步,但在HR病例中预防排斥反应仍然是难以捉摸的。本研究探讨血管内皮源性生长因子(VEGF)-C/D-VEGFR3通路在角膜移植中的作用,重点研究其对炎症和免疫反应的影响。方法:对42例患者42只眼进行评估,其中24例行角膜移植,分别于移植后90、180、360天进行随访。临床评估包括视力、角膜水肿、内皮细胞计数和血管形成。进行分子分析,检测VEGFR3、VEGF-C、VEGF-D、VEGF-A和炎症标志物。结果:结果显示,与低风险(LR)移植相比,HR移植中VEGF-C/D和VEGFR3的表达模式不同,与炎症标志物和临床结果相关。在HR病例中,VEGFR3表达升高与炎症标志物(细胞间粘附分子1 (ICAM-1)和人白细胞抗原-DR同型(HLA-DR))增加和移植物排斥风险相关。结论:这些发现强调了了解VEGF-C/D-VEGFR3通路在角膜移植,特别是HR病例中调节免疫反应和炎症的重要性。靶向这一途径可能为减轻炎症和提高移植物存活率提供新的治疗途径。需要进一步的研究来阐明潜在的机制并验证这些发现,潜在地提高移植结果,特别是在HR患者中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Ophthalmologica
Acta Ophthalmologica 医学-眼科学
CiteScore
7.60
自引率
5.90%
发文量
433
审稿时长
6 months
期刊介绍: Acta Ophthalmologica is published on behalf of the Acta Ophthalmologica Scandinavica Foundation and is the official scientific publication of the following societies: The Danish Ophthalmological Society, The Finnish Ophthalmological Society, The Icelandic Ophthalmological Society, The Norwegian Ophthalmological Society and The Swedish Ophthalmological Society, and also the European Association for Vision and Eye Research (EVER). Acta Ophthalmologica publishes clinical and experimental original articles, reviews, editorials, educational photo essays (Diagnosis and Therapy in Ophthalmology), case reports and case series, letters to the editor and doctoral theses.
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