Extended-spectrum beta-lactamase-producing Escherichia coli through the poultry production chain in Argentina

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-02-04 DOI:10.1016/j.vetmic.2025.110421

Juliana González , María Soledad Ríos , Luciana Hernandez , María Laura Chiapparrone , María Belén Riccio , Claudio Cacciato , Rocío Colello , Ana Victoria Bustamante , Andrea Mariel Sanso

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引用次数: 0

Abstract

The presence of extended-spectrum β-lactamases-producing Escherichia coli (ESBL-EC) in food production systems is a public health problem, since they can be transmitted to humans. The aims of this study were to isolate and characterize ESBL-EC from poultry farms located in Argentina. Resistances to β-lactams (first-, third-, and fourth-generation cephalosporins, penicillin), tetracyclines, quinolones, sulfonamides, phosphonates and aminoglycosides were found among the 40 ESBL-EC isolates. In addition, a wide range of antimicrobial resistance profiles were identified. The most frequent accompanying resistance to non-β-lactam antibiotics was tetracycline (97.5 %), followed by ciprofloxacin (82.5 %). All ESBL-EC isolates were multidrug resistant, and were grouped into four bla_CTX-M groups: bla_CTX-M-2 (75 %), followed by bla_CTX-M-1, bla_CTX-M-1/bla_CTX-M-2, and bla_CTX-M-2/bla_CTX-M-8. Of the 40 ESBL-EC isolates, 31 carried genes encoding integrase, 28 were positive for intI1- qacEΔ1- sul1, three for intI1, three were identified as Verocytotoxin-producing E. coli (VTEC) and most of the studied isolates belonged to the phylogroup E. This study reports the presence of CTX-M in E. coli obtained from poultry production in Argentina and that CTX-M-2 was the main mechanism responsible for third generation cephalosporins resistance. Also, showed that apparently healthy chickens in Argentina harbor commensal multidrug-resistant (MDR) ESBL-EC, with resistances to antibiotics of critical importance for human and veterinary medicine.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.