Detection of Trypanosoma cruzi in white-eared opossums (Didelphis albiventris) from Canoinhas, Santa Catarina State, Brazil1.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-02-03 eCollection Date: 2025-01-01 DOI:10.1590/S1984-29612025003

Giane Helenita Pontarolo, Daniela Pedrassani, Luís Felipe Kühl, Monique Paiva Campos, Thais Cristina Tirado, Fabiano Borges Figueiredo, Thállitha Samih Wischral Jayme Vieira, Ana Cláudia Calchi, Marcos Rogério André, Rafael Felipe da Costa Vieira, Ivan Roque de Barros Filho

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Abstract

Opossums are synanthropic animals that participate in the zoonotic transmission cycles. Chagas disease, a neglected tropical disease caused by Trypanosoma cruzi, affects many domestic and wild animals and humans worldwide. This study aimed to determine the occurrence of T. cruzi in free-ranging opossums in Canoinhas, Santa Catarina, Brazil. Fifty opossums (Didelphis albiventris) (33 captured and 17 road-killed) were evaluated using Nested-PCR assay. All tissue samples were negative (0/17). Eight of the 33 (24.24%; 95% CI:11.94-40,89%) blood samples were positive for T. cruzi. No significant associations were found between the sex (male/ female, p = 0.423), the trap area (rural/urban, p = 0.163), and positivity for T. cruzi in opossum blood samples. All samples showed 100% identity with T. cruzi (KF788250) isolated from Panstrongylus megistus in São Paulo, Brazil. The phylogenetic analysis model allocated all sequences obtained from D. albiventris to the large TcI clade of T. cruzi. This study provides the first record of T. cruzi in white-eared opossums in Canoinhas, Santa Catarina, southern Brazil.

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在巴西圣卡塔琳娜州卡诺因哈斯的白耳负鼠（Didelphis albiventris）中检测到克鲁兹锥虫1。

负鼠是参与人畜共患病传播循环的合群动物。恰加斯病是由克氏锥虫引起的一种被忽视的热带病，影响全世界许多家畜和野生动物以及人类。本研究旨在确定克氏锥虫在巴西圣卡塔琳娜州卡诺尼亚斯自由放养负鼠中的发生情况。采用巢式pcr法对50只负鼠（捕获33只，道路死亡17只）进行评价。所有组织样本均为阴性（0/17）。33人中有8人(24.24%；95% CI:11.94-40,89%)的血液样本呈克氏锥虫阳性。性别（男性/女性，p = 0.423）、诱捕区（农村/城市，p = 0.163）与负鼠血样克氏弓形虫阳性率无显著相关性。所有样本均与巴西圣保罗大圆形线虫分离株克氏t型虫（KF788250）同源性100%。系统发育分析模型将得到的所有序列均归属于克氏恙虫TcI大支系。本研究首次在巴西南部圣卡塔琳娜卡诺尼亚斯的白耳负鼠中发现克氏锥虫。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.