An insight into the potential antioxidant, anticancer and antimicrobial activities of Geitlerinema sp. C-phycocyanin extracts

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-01-01 DOI:10.1016/j.algal.2024.103836

Sona Hajiyeva , Meral Yılmaz Cankilic , Volkan Kilic , Sennur Gorgulu , Francesca Patrignani , Rosalba Lanciotti

{"title":"An insight into the potential antioxidant, anticancer and antimicrobial activities of Geitlerinema sp. C-phycocyanin extracts","authors":"Sona Hajiyeva , Meral Yılmaz Cankilic , Volkan Kilic , Sennur Gorgulu , Francesca Patrignani , Rosalba Lanciotti","doi":"10.1016/j.algal.2024.103836","DOIUrl":null,"url":null,"abstract":"<div><div>C-phycocyanin derived from cyanobacteria has recently attracted significant research attention due to its diverse bioactive compounds. The current research investigated the antioxidant and antimicrobial properties of lyophilized extracts from <em>Geitlerinema</em> sp., which exhibited partial purity (A<sub>620</sub>/A<sub>280</sub> = 2.78) in vitro. Additionally, we explored the anticancer effects of the C-phycocyanin extract of analytical grade (A<sub>620</sub>/A<sub>280</sub> = 4.12) which is purified through ion exchange chromatography.</div><div>We found an IC<sub>50</sub> value of 0.57 ± 0.01 mg/mL for C-phycocyanin, close to that of ascorbic acid (0.49 ± 0.08 mg/mL), suggesting its potential to be used as a specific antioxidant source. MTT assays on lung (A549, CCD-19Lu) and pancreas (Panc-1, hTERT-HPNE) cell lines provided IC<sub>50</sub> values which is lower for CCD-19Lu cells when compared to A529 cells and which is higher in Panc-1 cells when compared to hTERT-HPNE. As a result, the tested compound exhibited a favourable safety profile, with enhanced effectiveness at lower concentrations and significant anticancer activities against pancreatic cells after 72 h. Moreover, the compound was found to be effective antimicrobial action against both Gram-negative/Gram-positive bacteria and yeast. The results emphasize the promising bioactivity of C-phycocyanin in antimicrobial, antioxidant, and anticancer research.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"85 ","pages":"Article 103836"},"PeriodicalIF":4.6000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221192642400448X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

C-phycocyanin derived from cyanobacteria has recently attracted significant research attention due to its diverse bioactive compounds. The current research investigated the antioxidant and antimicrobial properties of lyophilized extracts from Geitlerinema sp., which exhibited partial purity (A₆₂₀/A₂₈₀ = 2.78) in vitro. Additionally, we explored the anticancer effects of the C-phycocyanin extract of analytical grade (A₆₂₀/A₂₈₀ = 4.12) which is purified through ion exchange chromatography.

We found an IC₅₀ value of 0.57 ± 0.01 mg/mL for C-phycocyanin, close to that of ascorbic acid (0.49 ± 0.08 mg/mL), suggesting its potential to be used as a specific antioxidant source. MTT assays on lung (A549, CCD-19Lu) and pancreas (Panc-1, hTERT-HPNE) cell lines provided IC₅₀ values which is lower for CCD-19Lu cells when compared to A529 cells and which is higher in Panc-1 cells when compared to hTERT-HPNE. As a result, the tested compound exhibited a favourable safety profile, with enhanced effectiveness at lower concentrations and significant anticancer activities against pancreatic cells after 72 h. Moreover, the compound was found to be effective antimicrobial action against both Gram-negative/Gram-positive bacteria and yeast. The results emphasize the promising bioactivity of C-phycocyanin in antimicrobial, antioxidant, and anticancer research.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment