Complete genome characterization of mcr-1-mediated colistin-resistant Escherichia coli from outpatients in Bulgaria

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Enfermedades infecciosas y microbiologia clinica Pub Date : 2025-02-01 DOI:10.1016/j.eimc.2024.10.002

Stefana Sabtcheva , Ivan Stoikov , Deyan Donchev , Sylvia Georgieva , Elina Dobreva , Ivan Nikolaev Ivanov

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Abstract

Introduction

This study reports the emergence of mcr-1-mediated colistin resistance in human Escherichia coli isolates from Bulgaria.

Methods

Three colistin-resistant E. coli isolates were obtained from outpatient urine specimens. They were subjected to PCR for detection of mcr genes and conjugation experiments. Whole-genome sequencing was employed to analyze the genomic characteristics of the isolates.

Results

PCR identified mcr-1 in all isolates. In E. coli of sequence type (ST) 2067, mcr-1.1 was found on a self-transmissible IncI2 plasmid, while mcr-1.32 was chromosomal in the remaining two ST131 E. coli isolates. E. coli ST2067 co-harbored quinolones resistance mutations (gyrA^D87N, gyrA^S83L, parC^S80I), β-lactam (bla_TEM-30) and aminoglycoside (aadA1, aac(3)-IId) resistance genes.

Conclusion

This report further confirms the role of IncI2 conjugative plasmids in the dissemination of mcr genes. Our findings involving chromosomal mcr-1 in high-risk ST131 E. coli strains from outpatients underscores the need for enhanced surveillance and systematic screening to combat colistin resistance.

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来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.