Anti-cancer potential of hydroethanolic extracts of Kyllinga nemoralis: An in vitro and in-silico studies

Eunice E. Ampem Danso , Justice Kumi , Abigail Aning , Sherif Hamidu , Janet Ampofo , Latif Adams , Isaac Asiamah , Francis Ackah Armah , Alexander K. Nyarko , Desmond Omane Acheampong
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Abstract

Introduction

This study aims to investigate the antioxidant and anti-proliferative properties of Kalinga nemoralis to regulate the production and application of herbal preparations.

Method

The leaves, roots and the whole plant extracts of Kyllinga nemoralis (KNL, KNR and KNW) were evaluated for antioxidant capacity, flavonoid content (TFC), phenolic content (TPC), in vitro cytotoxicity using Folin-Ciocalteu, Aluminium Chloride colorimetric methods and DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP assays respectively. LC-MS chemical profile was obtained by Agilent HPLC system.
The results obtained showed that, KNL extracts shown the highest TFC (16421.33 ± 0.06 mg QE/g) followed by KNW (10531 ± 0.02 mg QE/g) and KNR extracts (4741 ± 0.04 mg QE/g) with TFC/TPC ratio of 65.03, 58.50 and 23.75 respectively. The EC50 values of both DPPH and FRAP assays for the extracts ranged from 0.033 ± 0.07–4.10 ± 0.67 mg/mL. KNL and KNR had the strongest ferric reducing activity (EC50 values of 0.88 ± 0.20 mg/mL and 1.52 ± 0.36 mg/mL) while KNW had the least DPPH radical scavenging activity (2.48 ± 0.35 µg/mL). The IC50 value for each extract was greater than 1000 µg/mL, indicating minimal cytotoxicity against PNT2 cell line. KNR and KNW exhibited the strongest inhibitory activity against the PC3 cell line with an IC50 values of 52.65 ± 1.11 and 53.20 ± 0.80 µg/mL respectively. KNL exhibited a good inhibitory activity against the cancer cell line with an IC50 value of 68.54 ± 9.99. The selectivity indices of extracts were greater than 2. LC-MS profile showed four primary peaks representing combined plant constituents, with 12 recognized compounds and five undetermined compounds.

Conclusion

Kyllinga nemoralis demonstrates antioxidant activity and antiproliferative effects against PC3 cell lines, attributed to its bioactive compounds. Among these, Flufenacet, Diazoxide, and N-(2-Hydroxyphenyl)piperazine exhibited the highest drug-likeness scores with significant bioactivity.
线虫水乙醇提取物的抗癌潜力:体外和计算机研究
摘要本研究旨在研究卡林加的抗氧化和抗增殖活性,以指导中药制剂的生产和应用。方法采用福林比色法、氯化铝比色法、DPPH(2,2 -二苯基-1-苦酰肼)法和FRAP法,分别对线虫叶、根和全株提取物(KNL、KNR和KNW)的抗氧化能力、类黄酮含量(TFC)、酚类含量(TPC)和体外细胞毒性进行评价。采用安捷伦高效液相色谱系统进行色谱-质谱分析。获得的结果表明,KNL提取显示最高的交通(16421.33 ±0.06  mg QE / g)其次是认识(10531 ±0.02  mg QE / g)和KNR提取物(4741 ±0.04  mg QE / g)交通/ TPC比率为65.03,58.50和23.75。DPPH和FRAP的EC50值范围为0.033 ± 0.07-4.10 ± 0.67 mg/mL。KNL和KNR最强的铁减少活动(EC50值0.88 ±0.20  毫克/毫升和1.52 ±0.36  毫克/毫升)而认识至少DPPH自由基清除活性(2.48 ±0.35  µg / mL)。每种提取物的IC50值均大于1000 µg/mL,表明对PNT2细胞株的细胞毒性很小。KNR和KNW对PC3细胞株的抑制活性最强,IC50值分别为52.65 ± 1.11和53.20 ± 0.80 µg/mL。KNL对癌细胞具有良好的抑制活性,IC50值为68.54 ± 9.99。提取物的选择性指数均大于2。LC-MS图谱显示4个主峰代表组合植物成分,有12个已识别的化合物和5个未确定的化合物。结论线虫菌对PC3细胞株具有抗氧化和抗增殖作用,这与线虫菌的活性成分有关。其中,氟芬那塞、二氮氧化物和N-(2-羟基苯基)哌嗪表现出最高的药物相似性评分,具有显著的生物活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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