Phylogenetic annotation and expression analysis of heat shock protein 70 genes in two Henosepilachna species

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-11-29 DOI:10.1016/j.genrep.2024.102098

Yu-Xing Zhang , Hai-Hui Liu , Jia-Qing Yu , Lin Jin , Kai-Yun Fu , Wen-Chao Guo , Guo-Qing Li

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引用次数: 0

Abstract

Two Henosepilachna beetles are notorious defoliators mainly attacking potatoes. In China, H. vigintioctopunctata is predominantly distributed in the southern region of the Yangtze River, while H. vigintioctomaculata is found in northern area. In the current paper, we uncovered H. vigintioctopunctata displayed a higher thermotolerance than H. vigintioctomaculata. We identified 15 and 20 full-length heat shock protein 70 (hsp70) genes from H. vigintioctopunctata and H. vigintioctomaculata transcriptomes. A total of 8, 3, 2 and 2 Hvphsp70s in H. vigintioctopunctata, and 6, 9, 2 and 3 Hvmhsp70s in H. vigintioctomaculata fell into cytosolic A (hsp70ca), cytosolic B (hsp70cb), endoplasmic reticulum (hsp70era) and mitochondria (hsp70ma) subfamilies, respectively. Compared with H. vigintioctopunctata, two hspca genes (hspca6 and 7) were absent, whereas hsp70cb subfamily underwent independent expansion, resulting in the generation of 6 new hsp70cb members (Hvmhsp70cb4 to Hvmhsp70cb9) during evolution in H. vigintioctomaculata. Nine H. vigintioctopunctata hsp70s (Hvphsp70ca1-Hvphsp70ca7, Hvphsp70cb1 and Hvphsp70cb2) and 13H. vigintioctomaculata hsp70s (Hvmhsp70ca1-Hvmhsp70ca5, Hvmhsp70cb2, Hvmhsp70cb4-Hvmhsp70cb9 and Hvmhsp70era1) expressionally responded to heat shock, in a species-specific and temperature-dependent manner. Our findings raise a possibility that different members and/or specific compositions of hsp70s may cause thermoresistant disparity between the two Henosepilachna beetles.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.