{"title":"Exploring genetic diversity in apricot (Prunus armeniaca L.) populations using SSR markers","authors":"Bunty Shylla , Dharam Paul Sharma , Anil Handa , Manisha Thakur , Pooja Sharma , Narender Negi","doi":"10.1016/j.sajb.2024.11.030","DOIUrl":null,"url":null,"abstract":"<div><div>The genetic diversity of apricot (<em>Prunus armeniaca</em> L.) germplasm in mid-hills of Himachal Pradesh was investigated using Simple Sequence Repeats (SSR) markers. Apricot, a fruit-bearing tree known for its high nutritional value, requires genetic diversity analysis for effective breeding and conservation efforts. Molecular characterization using 46 SSR markers produced a total of 974 alleles with an average of 33.59 alleles per locus and PIC values ranging from 0.16 to 0.50, indicating a high level of polymorphism. PCR products of 15 indigenous collections and ‘Shakarpara’ revealed unique bands that were sequenced and subjected to BLAST, showing a high degree of homology with <em>Prunus dulcis.</em> A high quality reference genome for apricot is still unavailable, thereby limiting the elucidation of phenotypic associations with genetic background. Overall, this study provides valuable insights into the genetic diversity of apricot germplasm, crucial for sustainable breeding strategies, and need for integrating advanced genomic tools to further elucidate the genetic architecture of apricot.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"177 ","pages":"Pages 50-59"},"PeriodicalIF":2.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"South African Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254629924007476","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The genetic diversity of apricot (Prunus armeniaca L.) germplasm in mid-hills of Himachal Pradesh was investigated using Simple Sequence Repeats (SSR) markers. Apricot, a fruit-bearing tree known for its high nutritional value, requires genetic diversity analysis for effective breeding and conservation efforts. Molecular characterization using 46 SSR markers produced a total of 974 alleles with an average of 33.59 alleles per locus and PIC values ranging from 0.16 to 0.50, indicating a high level of polymorphism. PCR products of 15 indigenous collections and ‘Shakarpara’ revealed unique bands that were sequenced and subjected to BLAST, showing a high degree of homology with Prunus dulcis. A high quality reference genome for apricot is still unavailable, thereby limiting the elucidation of phenotypic associations with genetic background. Overall, this study provides valuable insights into the genetic diversity of apricot germplasm, crucial for sustainable breeding strategies, and need for integrating advanced genomic tools to further elucidate the genetic architecture of apricot.
期刊介绍:
The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.