Na Dong, Aiju Zhang, Xingde Dai, Xiaolin Zhang, Fangzhen He
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引用次数: 0
Abstract
Glucose oxidase can catalyze the reaction of glucose to produce hydrogen peroxide, and hydrogen peroxide reacts with excess I− anions in dilute sulfuric acid solution to form I3−. The I3− combines with Rhodamine 6G to form association which result in fluorescence quenching. Effect of glucose oxidase on fluorescence of Rhodamine 6G, a new method with high sensitivity and good selectivity for the determination of glucose oxidase activity, was established. Under the optimal conditions, the linear range of the method is 1.0 × 10–4–1.0 × 10–1 U/mL, and the detection limit is 9.0 × 10–5 U/mL. This assay was applied to the determination of glucose oxidase activity in the food additives samples with satisfactory results; the recoveries obtained were 96.67–99.00%.
Chemical PapersChemical Engineering-General Chemical Engineering
CiteScore
3.30
自引率
4.50%
发文量
590
期刊介绍：
Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.
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