Evaluation of toxicity and antioxidant activities of various crude extracts of leaves and stems of Zygophyllum simplex

Noha Maher Galal , Salem Said Al Touby , Yahya Bin Abdullah Alrashdi , Mohammad Amzad Hossain
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Abstract

Zygophyllum simplex (Z. simplex) is a plant that has been used for a long time for the treatment of human diseases. Therefore, this present research study aims to prepare various plant extracts and screen their antioxidant and cytotoxic activities. To attain the present objectives, different crude extracts were prepared from the leaves and stems of Z. simplex by using a maceration method. The activities of antioxidant and cytotoxic were prepared from aerial crude extracts of Z. simplex which were determined by 2,2-diphenyl-1-1-picrylhydrazyl (DPPH) and brine shrimp lethality (BSL) methods, respectively. All the prepared leaves and stems extracts of the selected plant at six different concentrations showed significant antioxidant activity against the DPPH method. The ethyl acetate crude extract showed the highest antioxidant activity and the lowest activity was in butanol extract. However, all the leaves and stems crude extracts of Z. simplex were prepared at different concentrations also showed promising cytotoxic activity against the BSL method. However, based on the antioxidant activity results, the ethyl acetate extract was selected for the isolation of bioactive compounds by using the column chromatographic method. The ethyl acetate was purified by using column chromatography in which different ratios of mobile phase (dichlorometane: methanol) were used. A series of test tubes were collected with a volume of 3 mL and depending on the similar retention mobility (Rf) behavior a total of twelve fractions were prepared. Similarly, the antioxidant activity of the obtained twelve fractions from column chromatography was determined by the same DPPH method. All the fractions showed significant antioxidant activity. Among the fractions from the column, fraction 6 give the highest antioxidant activity and the lowest was fraction 1. In conclusion, all the leaves and stems showed encouraging activities against DPPH and the fraction with the highest antioxidant activity could be used as a natural antioxidant to prevent cell damage.
小檗叶、茎不同粗提物的毒性及抗氧化活性评价
单纯合子(zz . simplex)是一种长期用于治疗人类疾病的植物。因此,本研究旨在制备各种植物提取物,并对其抗氧化和细胞毒活性进行筛选。为达到本研究目的,采用浸渍法制备了不同粗提物。采用2,2-二苯基-1-1-苦味酰肼(DPPH)法和卤虾致死性(BSL)法分别测定了其抗氧化活性和细胞毒活性。6种不同浓度的叶提取物和茎提取物对DPPH法均表现出显著的抗氧化活性。乙酸乙酯粗提物抗氧化活性最高,丁醇粗提物抗氧化活性最低。然而,不同浓度的单根叶和茎粗提物均表现出良好的细胞毒活性。然而,根据抗氧化活性结果,选择乙酸乙酯提取物,采用柱层析法分离生物活性化合物。采用不同比例的流动相(二氯甲烷:甲醇)柱层析法纯化乙酸乙酯。收集一系列体积为3ml的试管,根据相似的保留迁移率(Rf)行为,共制备了12个馏分。同样,用相同的DPPH法测定柱层析得到的12个组分的抗氧化活性。各组分均表现出显著的抗氧化活性。各馏分中抗氧化活性最高的是6,最低的是1。综上所述,所有叶和茎均表现出较强的抗DPPH活性,抗氧化活性最高的部位可作为天然抗氧化剂保护细胞免受损伤。
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来源期刊
Advances in biomarker sciences and technology
Advances in biomarker sciences and technology Biotechnology, Clinical Biochemistry, Molecular Medicine, Public Health and Health Policy
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