Effective detection of kanamycin residues by target recycling-based enzyme-free signal dual amplification electrochemical aptasensor

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI:10.1016/j.snb.2025.137365

Guochao Chen , Lingzhi Wang , Huan Liu , Lei Wang , Changyuan Yu , Hao Li

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Abstract

Abuse of kanamycin is still present in livestock and poultry farming, thereby causing serious damage to the human body through food chain. Therefore, it is necessary to effectively detect kanamycin in animal sourced food. Here, four different versions of electrochemical aptasensors were developed for efficient detection of kanamycin; especially, a target recycling-based enzyme-free signal dual amplification electrochemical aptasensor (i.e., named as Ver. 4 aptasensor) targeting kanamycin was developed. Under the optimized conditions, the Ver. 4 aptasensor exhibited a high sensitivity toward kanamycin with a limit of detection (LOD) of 0.10 ng/mL (linear range from 0.48 to 4844.99 ng/mL), and a high selectivity against the kanamycin’s structural analogs. Meanwhile, good reusability and excellent detection repeatability further indicated the application feasibility of the Ver. 4 aptasensor. Moreover, the recovery rate of kanamycin from spiked milk samples ranged from 93.24 % to 103.80 %. Results presented here suggested the application potential of the Ver. 4 aptasensor for effectively detecting kanamycin in animal sourced foods.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.