Oxytetracycline and Florfenicol Association Affects Zebrafish Larvae Behavioral Repertoire

IF 2.2 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Archives of Environmental Contamination and Toxicology Pub Date : 2025-01-28 DOI:10.1007/s00244-025-01113-0

Darlan Gusso, Marilia Oberto da Silva Gobbo, Gabriel Rübensam, Carla Denise Bonan

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Abstract

Oxytetracycline (OTC) and Florfenicol (FF) are prevalent antibiotics choices in both fish production and livestock farming. A comprehensive understanding of their effects is paramount for effective control of their use and for elucidating their physiological and pharmacological implications. In our investigation, zebrafish larvae were subjected to varying concentrations of OTC, FF or a combination of OTC + FF during 96 h. We observed behavioral alterations in the group exposed to OTC + FF. These fish displayed increased mobility, spent more time in the central zone, exhibited reduced turn angles, and experienced an impaired optomotor response. Coincidentally, our data provided evidence of reduced anxiety-like behavior in zebrafish larvae treated with OTC and FF, while also demonstrating the adverse effects of antibiotics on the optomotor response. Anxiety-like behavior plays an important role in species survival, acting as a key mechanism for adaptation and protection. The absence of such behavior can increase organism vulnerability in the environment. Thus, this study showed the behavioral consequences of OTC and FF exposure in zebrafish larvae, highlighting the impact of the combined toxicity of these antibiotics.

Graphical Abstract

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土霉素和氟苯尼考联用对斑马鱼幼虫行为的影响

土霉素（OTC）和氟苯尼考（FF）是鱼类生产和畜牧业中普遍使用的抗生素。全面了解其作用对于有效控制其使用和阐明其生理和药理学意义至关重要。在我们的研究中，斑马鱼幼虫在96小时内受到不同浓度的OTC、FF或OTC + FF组合的影响。我们观察了暴露于OTC + FF组的行为改变。这些鱼表现出更强的活动性，花更多的时间在中心区域，表现出更小的转角，并且经历了受损的视运动反应。巧合的是，我们的数据提供了证据，证明使用OTC和FF治疗的斑马鱼幼虫的焦虑样行为减少，同时也证明了抗生素对视运动反应的不利影响。类焦虑行为在物种生存中起着重要作用，是适应和保护的关键机制。缺乏这种行为会增加生物体在环境中的脆弱性。因此，本研究显示了OTC和FF暴露在斑马鱼幼虫中的行为后果，突出了这些抗生素联合毒性的影响。

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来源期刊

Archives of Environmental Contamination and Toxicology 环境科学-毒理学

CiteScore

7.00

自引率

2.50%

发文量

审稿时长

8-16 weeks

期刊介绍： Archives of Environmental Contamination and Toxicology provides a place for the publication of timely, detailed, and definitive scientific studies pertaining to the source, transport, fate and / or effects of contaminants in the environment. The journal will consider submissions dealing with new analytical and toxicological techniques that advance our understanding of the source, transport, fate and / or effects of contaminants in the environment. AECT will now consider mini-reviews (where length including references is less than 5,000 words), which highlight case studies, a geographic topic of interest, or a timely subject of debate. AECT will also consider Special Issues on subjects of broad interest. The journal strongly encourages authors to ensure that their submission places a strong emphasis on ecosystem processes; submissions limited to technical aspects of such areas as toxicity testing for single chemicals, wastewater effluent characterization, human occupation exposure, or agricultural phytotoxicity are unlikely to be considered.