Camille Daviaud, María Cecilia Lira, Claire Vanpouille-Box, Mara De Martino
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引用次数: 0
Abstract
Glioblastomas (GBMs) are the most common and aggressive brain tumors, with a poor prognosis. Effective preclinical models are crucial to investigate GBM biology and develop novel treatments. Syngeneic models, which consist in injecting murine GBM cells into mice with a similar genetic background, offer reproducibility, cost-effectiveness, and an intact immune system, making them ideal for immunotherapy research. This chapter presents a comprehensive protocol for stereotactic injection of murine GBM cells into immunocompetent mice to induce intracranial GBM. The protocol covers cell culture, anesthesia, surgical procedures, and post-operative care, allowing the reliable induction of orthotopic brain tumors. This method can be used to study anti-GBM therapies, including immunotherapies, and has the potential to accelerate the development of effective treatments.
期刊介绍：
For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
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