In Vitro Biochemical Control of Taenia solium and Taenia saginata Eggs

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-01-24 DOI:10.1007/s11686-024-00950-8

Debora Castro de Souza, Lisseth Bibiana Figueroa Puentes, Adriane Toledo da Silva, Dyesse Pollyane Ferreira, Carolina Magri Ferraz, Fabio Ribeiro Braga, Filippe Elias de Freitas Soares

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引用次数: 0

Abstract

Purpouse

The present study evaluated in vitro the action of the plant protease papain (EC 3.4.22.2) on intact eggs of Taenia solium and Taenia saginata.

Methods

Two experimental groups were composed for each of the gastrointestinal helminths: control group (G1) and treated group (G2). In each replicate corresponding to the control group, 100 µL of the suspension containing approximately 100 intact eggs (T. solium) or 50 eggs (T. saginata) and 100 µL of distilled water were added, while for the treated group 100 µL of the egg suspension and 100 µL of the papain solution at a concentration of 15% (m/v) were added. The experiment was conducted in four replicates. The groups were incubated in BOD at 28 ± 1 ºC in the dark for a period of 24 h.

Results

The results demonstrated that there was a significant reduction (p < 0.01) of intact eggs, with average reduction percentages for T. saginata and T. solium of 74.36% and 64.29%, respectively.

Conclusions

This study is the first report on the in vitro control of helminth eggs (T. saginata and T. solium) using pure papain.

Abstract Image

查看原文本刊更多论文

猪带绦虫和牛带绦虫卵的体外生化控制。

目的：研究植物蛋白酶木瓜蛋白酶（EC 3.4.22.2）对猪带绦虫（Taenia solium）和牛带绦虫（Taenia saginata）完整卵的体外作用。方法：将胃肠道蠕虫分为对照组（G1）和治疗组（G2）两组。在对照组对应的每个重复中，加入100µL含有约100个完整卵（T. solium）或50个完整卵（T. saginata）的悬浮液和100µL蒸馏水，而在处理组中加入100µL卵悬浮液和100µL浓度为15% （m/v）的木瓜蛋白酶溶液。试验分4个重复进行。结果：结果表明，用纯木瓜蛋白酶体外控制蠕虫卵（T. saginata和T. solium）的研究尚属首次报道。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.