Genome-Wide Analysis of the CsAP2/ERF Gene Family of Sweet Orange (Citrus sinensis) and Joint Analysis of Transcriptional Metabolism under Salt Stress.
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引用次数: 0
Abstract
Background: Sweet orange is an important economic crop, and salt stress can inhibit its growth and development.
Methods: In this study, we identified AP2/ERF genes in sweet orange via bioinformatics and performed a combined transcription‒metabolism analysis, which revealed for the first time the integrated molecular mechanism of salt stress regulation in sweet orange.
Key results: A total of 131 sweet orange AP2/ERF genes were identified and categorized into five groups. By comparing the tertiary protein structures of these genes with those of Arabidopsis, we found that five sweet orange genes (CsERF38, CsERF41, CsERF42, CsERF84, and CsERF110) related to salt stress and ethylene transcription are highly similar in composition and structure to those of Arabidopsis, and we hypothesize that they have similar functions. ABREs and AREs were the predominant cis-acting elements in the sweet orange AP2/ERF gene family, and both were associated with salt stress. The AP2/ERF gene family was verified to be involved in the salt stress response via qRT-PCR. According to the differentially abundant metabolite KEGG network, we chose the differentially abundant metabolites ET, GA, and JA as the primary research objects; the CsAP2/ERF gene family is an ethylene-responsive element binding factor.
Conclusion: In this study, the complete framework of the AP2/ERF gene family was constructed for the first time. A model of salt stress regulation in sweet oranges was established.
期刊介绍：
Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide.
The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.
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