Protocol to perform ex vivo electroretinography on adult zebrafish.

Abstract

Ex vivo electroretinography (ERG) provides insight into the health and functionality of retinal cells, the integrity of phototransduction, and the visual cycle and allows for the direct application of pharmaceuticals to the retinal tissues. Here, we present a protocol for performing ex vivo ERG on adult zebrafish. We describe steps for zebrafish tissue dissection, mounting tissues, and assembling and connecting cassettes. We then detail procedures for running the Diagnosys software and processing and analyzing the resulting data.