Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates.

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-01-20 DOI:10.1016/j.sbi.2024.102976

Alexander I M Sever, Rashik Ahmed, Philip Rößler, Lewis E Kay

引用次数: 0

Abstract

The tools of structural biology have undergone remarkable advances in the past decade. These include new computational and experimental approaches that have enabled studies at a level of detail - and ease - that were not previously possible. Yet, significant deficiencies in our understanding of biomolecular function remain and new challenges must be overcome to go beyond static pictures towards a description of function in terms of structural dynamics. Solution Nuclear Magnetic Resonance (NMR) spectroscopy has emerged as a powerful technique for atomic resolution studies of the dynamics of a wide range of biomolecules, including molecular machines and the components of phase-separated condensates. Here we highlight some of the very recent advances in these areas that have been driven by NMR.

查看原文本刊更多论文

溶液核磁共振大：分子机器和相分离凝聚物的蛋白质组分的原子分辨率研究。

结构生物学的工具在过去十年中取得了显著的进步。其中包括新的计算和实验方法，这些方法使研究达到了以前不可能做到的细节和简单程度。然而，我们对生物分子功能的理解仍然存在重大缺陷，必须克服新的挑战，以超越静态图片，以结构动力学的方式描述功能。溶液核磁共振（NMR）光谱已经成为一种强大的技术，用于广泛的生物分子动力学的原子分辨率研究，包括分子机器和相分离凝聚物的组成。在这里，我们将重点介绍由核磁共振驱动的这些领域的一些最新进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation