{"title":"Enrichment and purification of trace substances from yew extracum by twin-column recycling chromatography with a step solvent gradient.","authors":"Wei Xie, Yuxue Wu, Guangxia Jin, Jiarong Sang, Feng Wei","doi":"10.1016/j.jchromb.2025.124467","DOIUrl":null,"url":null,"abstract":"<p><p>The concentration and purification of trace substances from natural products represent a bottleneck in current chromatographic separations. In this study, a twin-column recycling chromatography with a step solvent gradient method was proposed and successfully applied in the purification of unknown substances with tiny content in yew extracum. The method consists of two steps. Initially, the target substances were enriched from the yew extracum stock solution, followed by further separation and purification of the enriched substances in the second step. Ultimately, the target substances A and B were obtained with a purity of 99.7 % and 96.6 %, respectively. The proposed method has the potential to be applied in the preparation of trace substances for toxicological evaluation in natural product studies.</p>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1252 ","pages":"124467"},"PeriodicalIF":2.8000,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography B","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1016/j.jchromb.2025.124467","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
The concentration and purification of trace substances from natural products represent a bottleneck in current chromatographic separations. In this study, a twin-column recycling chromatography with a step solvent gradient method was proposed and successfully applied in the purification of unknown substances with tiny content in yew extracum. The method consists of two steps. Initially, the target substances were enriched from the yew extracum stock solution, followed by further separation and purification of the enriched substances in the second step. Ultimately, the target substances A and B were obtained with a purity of 99.7 % and 96.6 %, respectively. The proposed method has the potential to be applied in the preparation of trace substances for toxicological evaluation in natural product studies.
期刊介绍：
The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis.
Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches.
Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.
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