Genetic diversity and virulence gene profiling of Vibrio harveyi in a vibriosis-affected European seabass (Dicentrarchus labrax) aquaculture tank

IF 5.3 3区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Marine pollution bulletin Pub Date : 2025-01-16 DOI:10.1016/j.marpolbul.2025.117553

A. Da Fonseca Ferreira, A. Lehmann, T. Grard, R. Roquigny, C. Le Bris

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引用次数: 0

Abstract

Aquaculture is crucial for meeting global seafood demand; however, intensification often leads to the development of bacterial diseases that threaten productivity. Dicentrarchus labrax, a key species in European aquaculture, is highly vulnerable to vibriosis, primarily caused by Vibrio harveyi. This study investigates genetic diversity of V. harveyi isolates collected from a seabass tank affected by vibriosis. Sampling from biofilm and water environments yielded 946 bacterial isolates, of which 56 were identified as V. harveyi using MALDI-TOF MS. ERIC-PCR genotyping revealed four distinct profiles. Despite observing variability in the presence of the 80 virulence genes tested, the overall genetic variation among these profiles was not pronounced. The aim of this study was to determine if the presence of any V. harveyi environmental genotype profile could be used as an early predictor of vibriosis outbreak. Notably, no single environmental genotypic profile was linked to this latter. These findings suggest that the presence of virulence genes alone may not predict disease outbreaks, thus underscoring the need for future research on environmental and transcriptional factors that influences the virulence and pathogenicity to improve disease control in aquaculture systems.

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欧洲鲈鱼弧菌病养殖池中哈维弧菌的遗传多样性和毒力基因分析

水产养殖对满足全球海产品需求至关重要；然而，集约化养殖往往会导致细菌性疾病的发生，从而威胁生产力。欧洲水产养殖业中的一个重要物种--马口鱼，极易感染主要由哈维氏弧菌引起的弧菌病。本研究调查了从受弧菌病影响的鲈鱼养殖池中采集的 Harveyi弧菌分离物的遗传多样性。从生物膜和水环境中采样后，共分离出 946 个细菌，其中 56 个通过 MALDI-TOF MS 鉴定为 Harveyi弧菌。ERIC-PCR 基因分型显示出四种不同的特征。尽管在检测的 80 个毒力基因中发现了差异，但这些特征之间的总体遗传变异并不明显。本研究的目的是确定是否存在任何哈维氏弧菌环境基因型特征可作为弧菌病爆发的早期预测指标。值得注意的是，没有任何一种环境基因型特征与后者有关。这些研究结果表明，仅凭毒力基因的存在可能无法预测疾病的爆发，因此今后需要对影响毒力和致病性的环境和转录因子进行研究，以改善水产养殖系统的疾病控制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine pollution bulletin 环境科学-海洋与淡水生物学

CiteScore

10.20

自引率

15.50%

发文量

1077

审稿时长

68 days

期刊介绍： Marine Pollution Bulletin is concerned with the rational use of maritime and marine resources in estuaries, the seas and oceans, as well as with documenting marine pollution and introducing new forms of measurement and analysis. A wide range of topics are discussed as news, comment, reviews and research reports, not only on effluent disposal and pollution control, but also on the management, economic aspects and protection of the marine environment in general.