Radiopaque hydrogel-in-liposomes towards theranostic applications for malignant tumors

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2025-02-01 DOI:10.1016/j.biopha.2025.117822

Sang Min Lee , So-Yeol Yoo , Taejung Kim , Nahyun Kim , JungHun Kang , Ka-Young Lim , Minkyung Kim , Suwon Choo , Han Sol Lee , Hyelim Kim , Nae-Won Kang , Mansingh Chaudhary , Khadka Bikram , Wonhwa Lee , Cheong-Weon Cho , Dae-Duk Kim , Ki-Taek Kim , Jae-Young Lee

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引用次数: 0

Abstract

A radiopaque hydrogel-in-liposome (RHL) system was developed for micro-computed tomography (μCT) imaging of tumor tissue and simultaneous delivery of a cytotoxic agent. Iopamidol (IPD) and doxorubicin (DOX) were incorporated as the CT contrast and anti-cancer agents, respectively. The presence of a polyethylene glycol hydrogel core in the liposomes was confirmed via attenuated total reflectance Fourier transform infrared, proton nuclear magnetic resonance, and selective solvent extraction. Nano-sized (∼160 nm) spherical DOX/IPD-loaded RHLs with a narrow size distribution and negative zeta potential were successfully fabricated. The RHLs demonstrated enhanced cellular uptake of DOX in SCCVII cells compared to conventional radiopaque liposomes, likely due to clathrin-mediated endocytosis. Additionally, pH-dependent DOX release and reduced IPD leakage were observed. In vivo studies using SCCVII tumor-xenografted mice showed that RHLs exhibited improved CT contrast efficiency and anti-tumor efficacy, along with systemic biocompatibility, attributed to enhanced tumor-targeting properties. These findings suggest that the RHL system could serve as a promising nano-platform for tumor theranostics.

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用于恶性肿瘤治疗的不透射线脂质体水凝胶。

研制了一种不透射线的脂质体水凝胶（RHL）系统，用于肿瘤组织的微计算机断层扫描（μCT）成像和同时递送细胞毒性药物。分别采用Iopamidol （IPD）和doxorubicin （DOX）作为CT造影剂和抗癌剂。通过衰减全反射傅里叶变换红外、质子核磁共振和选择性溶剂萃取证实了脂质体中存在聚乙二醇水凝胶核。成功制备了具有窄尺寸分布和负zeta电位的纳米（~ 160 nm）球形DOX/ ipd负载rhl。与传统的不透射线脂质体相比，rhl显示SCCVII细胞对DOX的细胞摄取增强，可能是由于网格蛋白介导的内吞作用。此外，观察到ph依赖性DOX释放和IPD泄漏减少。使用SCCVII肿瘤异种移植小鼠进行的体内研究表明，rhl由于增强的肿瘤靶向特性，具有更好的CT造影剂效率和抗肿瘤功效，以及系统生物相容性。这些发现表明，RHL系统可以作为一个有前途的肿瘤治疗纳米平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.