Exploring high molecular weight components in Tityus serrulatus venom.

IF 2.6 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Nicoly Malachize Alano-da-Silva, Isadora Sousa de Oliveira, Iara Aimê Cardoso, Karla de Castro Figueiredo Bordon, Eliane Candiani Arantes
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引用次数: 0

Abstract

Our study identified high-molecular-weight compounds from Tityus serrulatus venom (TsV), and most of them have not yet been well explored. TsV was fractionated using FPLC system with different columns, analyzed by SDS-PAGE, and characterized by MALDI-TOF/TOF. Our study showed that TsV contains several high-molecular-weight compounds, including CRISPs, metalloproteinase and hyaluronidase. We show how these molecules can be obtained from TsV, enabling future studies about their molecular structures and biological actions, expanding knowledge about this venom.

探索蛇毒中的高分子量成分。
本研究鉴定出了一些高分子量化合物,其中大部分尚未被充分开发。采用不同色谱柱的FPLC分离TsV, SDS-PAGE分析,MALDI-TOF/TOF表征。我们的研究表明,TsV含有多种高分子量化合物,包括CRISPs、金属蛋白酶和透明质酸酶。我们展示了如何从TsV中获得这些分子,从而使未来对其分子结构和生物作用的研究成为可能,扩大了对这种毒液的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxicon
Toxicon 医学-毒理学
CiteScore
4.80
自引率
10.70%
发文量
358
审稿时长
68 days
期刊介绍: Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.
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