Investigating causal relationships between plasma proteins and lung adenocarcinoma: result from proteomics and Mendelian randomization study.

IF 2.8 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Discover. Oncology Pub Date : 2025-01-13 DOI:10.1007/s12672-025-01778-y

Ruoya Lv, Jiabin Chen, Xiaoyu Wu, Kequn Chai, Jiadong Yan, Sheng Wang

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Abstract

Background: Plasma proteins contribute to the identification, diagnosis, and prognosis of human illnesses, which may be conducive to understanding the molecular mechanism and diagnosis of Lung adenocarcinoma (LUAD).

Methods: We collected plasma samples from 28 healthy individuals (H) and 56 LUAD patients and analyzed them using LC-MS/MS-based proteomics to determine differential expression plasma proteins (DEPPs). Then, the DEPPs were subjected to a two-sample Mendelian randomization (MR) study based on an "Inverse variance weighted (IVW)" approach to investigate the causal relationships between DEPPs and LUAD. Logistic regression analysis was conducted to develop a diagnostic model for LUAD.

Results: 317 plasma proteins were found in proteomics, and 19 DEPPs were identified. The MR study revealed that IL20RB (odds ratio (OR) = 1.600, 95% Confidence Interval (CI) [1.098, 2.331], P = 0.014) and SAA2 (OR = 1.048, 95%CI [1.012, 1.081], P = 0.017) were highly related to LUAD. A diagnostic model was established with IL20RB and SAA2. The AUC of this diagnostic model was 0.858.

Conclusion: Plasma IL20RB and SAA2 levels were closely connected with LUAD.

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研究血浆蛋白与肺腺癌之间的因果关系：蛋白质组学和孟德尔随机化研究的结果。

背景：血浆蛋白有助于人类疾病的识别、诊断和预后，可能有助于了解肺腺癌（LUAD）的分子机制和诊断。方法：收集28例健康个体(H)和56例LUAD患者的血浆样本，采用LC-MS/MS-based蛋白质组学分析血浆差异表达蛋白（DEPPs）。然后，对DEPPs进行基于“逆方差加权（IVW）”方法的双样本孟德尔随机化（MR）研究，以调查DEPPs与LUAD之间的因果关系。采用Logistic回归分析建立LUAD诊断模型。结果：在蛋白质组学中发现317个血浆蛋白，鉴定出19个DEPPs。MR研究显示，IL20RB(比值比（OR) = 1.600, 95%可信区间（CI） [1.098, 2.331], P = 0.014）和SAA2 （OR = 1.048, 95%CI [1.012, 1.081], P = 0.017）与LUAD高度相关。用IL20RB和SAA2建立诊断模型。该诊断模型的AUC为0.858。结论：血浆IL20RB、SAA2水平与LUAD密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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