Dynamic Responses in Bioaugmentation of Petroleum-Contaminated Soils using Thermophilic Degrading Consortium HT: Hydrocarbons, Microbial Communities, and Functional Genes

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-01-14 DOI:10.1016/j.jhazmat.2025.137222

Qingling Wang, Jinyu Hou, Li Peng, Wuxing Liu, Yongming Luo

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Abstract

Bioaugmentation offers an effective strategy for the bioremediation of petroleum-contaminated soils. However, little is known about petroleum hydrocarbons (PHs) degradation with thermophilic consortium application under high temperature. A microcosm was established to study hydrocarbons degradation, microbial communities and functional genes response using a thermophilic petroleum-degrading consortium HT. The results showed that the consortium HT significantly enhanced PHs degradation, particularly for medium (C16-C21) (87.1%) and long-chain alkanes (C21-C40) (67.2%) within 140 days under high temperature. Colonization of HT in the soil exhibited lagged characteristics, with a substantial increase in bacterial genera originated from the HT after 60 days. Additionally, LEfSe analysis indicated that the biomarkers of HT treatment were mainly from the HT consortium. Moreover, functional analysis revealed genes related to n-alkane degradation (AlkB, P450, LadA), alkane utilization regulator (AraC, TetR, GntR), as well as several thermotolerance genes were significantly increased in HT treatment. Additionally, network analysis demonstrated distinct co-occurrence patterns induced by nutrient addition and exogenous consortium, with the latter strengthening interactions and stability of bacterial networks under high temperature. This study represents pioneering investigation into the effects of exogenous thermophilic consortium on petroleum degradation, bacterial communities, functional genes and ecological interactions in application of petroleum remediation under thermophilic conditions.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.