Chronic fat consumption affects metabolic aspects of murine physiology and influences ovarian follicle atresia

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2025-01-10 DOI:10.1016/j.reprotox.2025.108836

Ayodeji Ojo Oteyola , Isadora Maria Sátiro de Oliveira , Jonathas Medeiros de Almeida , Lucas Carvalho Cardoso , Thais de Merici Domingues e Paula , Julia Meireles Nogueira , Jade Carceroni de Sousa Carvalho , Henrique Martins Nogueira , Brenda Lee Simas Porto , Ana Paula Lucas Mota , Paulo Henrique Almeida Campos-Junior , Erika Cristina Jorge , Fernanda Radicchi Campos Lobato de Almeida

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引用次数: 0

Abstract

Saturated fat has been linked to cardiovascular diseases, leading to an increase in polyunsaturated fat consumption. The aim of the present study was to investigate the effects of three fat sources (coconut oil, lard and soybean oil) on metabolic and reproductive parameters in heterogenic mice. Female Swiss mice (5–6 weeks old; n = 9/group) were divided into four experimental groups: control (CC), coconut oil (CO), lard (LA), and soybean oil (SO), and were orally given 0.6 mL of the corresponding fat daily for 6 weeks to further investigate morphological, biochemical, and molecular parameters. SO females showed the highest glucose intolerance (P < 0.05), and all experimental groups were highly insulin resistant, with no statistical differences among them (P > 0.05). Moreover, all fat supplemented groups presented increased proportion in bile ducts, and CO and SO females presented higher LDL-cholesterol levels compared to CC (P < 0.05). Regarding reproductive parameters, estrous cycle alterations were observed mainly in the SO group, showing extended luteal phase duration (longer diestrus), and higher numbers of atretic follicles per area compared to the CC females (P < 0.05). Furthermore, higher proportion of active Casp-3 positive cells in the granulosa layers of preantral follicles were observed in the LA compared to the CO group (P < 0.05). The gene expression data revealed downregulation of Igf1r and Acvr1 in SO, upregulation of Igf1r in LA and Lhcgr in CO compared to CC females (P < 0.05). Thus, excessive fat intake may have deleterious effects on metabolism and reproductive function, but lard may be the least harmful source.

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慢性脂肪消耗影响小鼠生理代谢方面并影响卵巢卵泡闭锁。

饱和脂肪与心血管疾病有关，导致多不饱和脂肪消耗增加。本研究的目的是研究三种脂肪来源（椰子油、猪油和大豆油）对异种小鼠代谢和生殖参数的影响。雌性瑞士小鼠(5-6周龄；n=9/组)分为对照组（CC）、椰子油组（CO）、猪油组（LA）和大豆油组（SO） 4个实验组，每天口服相应脂肪0.6mL，持续6周，进一步研究形态学、生化和分子参数。SO女性表现出最高的葡萄糖耐受不良（P0.05）。此外，所有脂肪补充组的胆管比例均增加，CO和SO女性的ldl -胆固醇水平均高于CC (P

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来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.