Mouse Intestinal Organoid Culture Protocol.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-09 DOI:10.1007/7651_2024_591

Özüm Begüm Böke, Ezgi Bulut-Okumuş, Hazar Eren Soydan, Selinay Şenkal-Turhan, Ayşegül Doğan

引用次数: 0

Abstract

The discovery of leucine-rich-containing G-protein-coupled receptor 5 (Lgr5) as an intestinal adult stem cell marker had blazed a trail in stem cell biology and laid the foundations of modern organoid technology. Up to date, several well-established intestinal organoid protocols have been reported in the literature from different sources, including adult and induced pluripotent stem cells. Here, we demonstrate a BALB/c mouse-derived intestinal organoid culture protocol, passaging, and cryopreservation procedures.

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小鼠肠道类器官培养方案。

富含亮氨酸的g蛋白偶联受体5 （Lgr5）作为肠道成体干细胞标志物的发现，开辟了干细胞生物学的新途径，奠定了现代类器官技术的基础。到目前为止，文献中已经报道了几种成熟的肠道类器官方案，包括成体和诱导多能干细胞。在这里，我们展示了一种BALB/c小鼠来源的肠道类器官培养方案、传代和冷冻保存程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.