Light-/pH-Regulated Spiropyran Smart-Responsive Hydrophilic Separation Platform for the Identification of Serum Glycopeptides from Hepatocellular Carcinoma Patients

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-07 DOI:10.1021/acs.analchem.4c04025

Yanqing Zhao, Hongbin Li, Haijiao Zheng, Qiong Jia

{"title":"Light-/pH-Regulated Spiropyran Smart-Responsive Hydrophilic Separation Platform for the Identification of Serum Glycopeptides from Hepatocellular Carcinoma Patients","authors":"Yanqing Zhao, Hongbin Li, Haijiao Zheng, Qiong Jia","doi":"10.1021/acs.analchem.4c04025","DOIUrl":null,"url":null,"abstract":"Smart-responsive materials have attracted much attention in the enrichment of post-translational modifications of proteins. In this work, for the first time, we developed a smart enrichment strategy (MNPs-l-DOPA/PEI-SP) based on the change in hydrophilic properties of spiropyran under the regulation of light and pH to realize the controllable enrichment and release of intact glycopeptides. The enrichment mechanism and possible binding mechanism were verified by theoretical calculations. The smart enrichment platform based on MNPs-l-DOPA/PEI-SP was used to screen glycoprotein biomarkers for hepatocellular carcinoma (HCC) to evaluate its cancer diagnostic and monitoring performance. A total of 3,864 intact N-glycopeptides containing 166 N-glycoproteins were successfully identified in serum samples of early-stage HCC patients, while 3,266 intact N-glycopeptides containing 193 glycoproteins were identified in normal control (NC) serum samples. This work not only provides new ideas for the efficient enrichment of intact glycopeptides with smart-responsive material, but also broadens the research possibilities for biomarker discovery in HCC serum liquid biopsies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"82 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c04025","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Smart-responsive materials have attracted much attention in the enrichment of post-translational modifications of proteins. In this work, for the first time, we developed a smart enrichment strategy (MNPs-l-DOPA/PEI-SP) based on the change in hydrophilic properties of spiropyran under the regulation of light and pH to realize the controllable enrichment and release of intact glycopeptides. The enrichment mechanism and possible binding mechanism were verified by theoretical calculations. The smart enrichment platform based on MNPs-l-DOPA/PEI-SP was used to screen glycoprotein biomarkers for hepatocellular carcinoma (HCC) to evaluate its cancer diagnostic and monitoring performance. A total of 3,864 intact N-glycopeptides containing 166 N-glycoproteins were successfully identified in serum samples of early-stage HCC patients, while 3,266 intact N-glycopeptides containing 193 glycoproteins were identified in normal control (NC) serum samples. This work not only provides new ideas for the efficient enrichment of intact glycopeptides with smart-responsive material, but also broadens the research possibilities for biomarker discovery in HCC serum liquid biopsies.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.