Microplastics can alter structural configurations of human non-canonical G-quadruplex DNA

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2025-01-04 DOI:10.1016/j.etap.2025.104626

Sagar Bag, Souvik Ghosal, Sudipta Bhowmik

引用次数: 0

Abstract

Microplastics (MP) with a diameter of less than 150 μm can enter the lymph and bloodstream systems, induce cellular toxicity and damage DNA. G-quadruplexes (GQs) are tetraplex DNA secondary structures found in the human genomes that play important roles in replication, transcription and genomic integrity. Comprehending the biological and molecular processes underlying the activities of MPs could aid in estimating potential hazards to humans. In this investigation, polystyrene microplastics (PS-MPs) and polyethylene microplastics (PE-MPs) have been selected because these two MPs are environmentally most prevalent and they are of different sizes. Several biophysical strategies were employed to identify that PS-MPs are the most potent MPs that bind to CMYC GQ DNA (present in the promoter of CMYC gene, important for cellular growth and proliferation) and may alter their structure. This study helps to understand the potential threat MPs possesses by interacting with key DNA structures.

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微塑料可以改变人类非规范g -四重体DNA的结构构型

直径小于150 μm的微塑料（MP）可以进入淋巴和血液系统，引起细胞毒性并损伤DNA。g -四重体（GQs）是人类基因组中发现的四重体DNA二级结构，在复制、转录和基因组完整性中起着重要作用。了解MPs活动背后的生物学和分子过程有助于估计对人类的潜在危害。在本研究中，选择聚苯乙烯微塑料（PS-MPs）和聚乙烯微塑料（PE-MPs），因为这两种微塑料在环境中最普遍，而且它们的尺寸不同。采用了几种生物物理策略来确定PS-MPs是结合CMYC GQ DNA（存在于CMYC基因的启动子中，对细胞生长和增殖很重要）并可能改变其结构的最有效的MPs。这项研究有助于了解MPs通过与关键DNA结构相互作用而具有的潜在威胁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.