LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1.

IF 3.8 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-01-06 DOI:10.1038/s41598-025-85321-7

Xiao-Qian Wang, A-Qiang Fan, Liu Hong

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引用次数: 0

Abstract

This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and examined the effects of its overexpression and knockdown on cell proliferation. Proteomic analysis was conducted to explore the interaction between MIR210HG and ferroptosis pathway components. The binding of MIR210HG to poly(rC) binding protein 1 (PCBP1) was predicted using catRAPID and confirmed through RNA pull-down and RNA immunoprecipitation (RIP) experiments. MIR210HG was significantly upregulated in colon cancer cells and tissues. Its overexpression promoted, while its knockdown inhibited, colon cancer cell proliferation. MIR210HG was found to be associated with ferroptosis pathway components and to bind to PCBP1, which was experimentally validated. The inhibition of ferroptosis by MIR210HG through PCBP1 binding was confirmed, highlighting its role in promoting cell proliferation. MIR210HG promotes colon cancer cell proliferation by binding to PCBP1 and inhibiting ferroptosis. These findings suggest MIR210HG as a potential therapeutic target for colon cancer.

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本研究旨在探究长非编码 RNA（lncRNA）MIR210 宿主基因（MIR210HG）在结肠癌细胞增殖中的作用及其涉及铁突变通路的潜在机制。我们评估了 MIR210HG 在结肠癌细胞系和组织中的表达，并研究了其过表达和敲除对细胞增殖的影响。我们还进行了蛋白质组学分析，以探究 MIR210HG 与铁凋亡通路成分之间的相互作用。利用 catRAPID 预测了 MIR210HG 与多（rC）结合蛋白 1（PCBP1）的结合，并通过 RNA 拉取和 RNA 免疫沉淀（RIP）实验进行了证实。MIR210HG 在结肠癌细胞和组织中明显上调。MIR210HG在结肠癌细胞和组织中明显上调，过表达促进结肠癌细胞增殖，而敲除则抑制细胞增殖。实验发现 MIR210HG 与铁凋亡通路成分相关，并与 PCBP1 结合。证实了 MIR210HG 通过与 PCBP1 结合抑制铁凋亡，突出了它在促进细胞增殖方面的作用。MIR210HG 通过与 PCBP1 结合并抑制铁凋亡促进结肠癌细胞增殖。这些发现表明 MIR210HG 是结肠癌的潜在治疗靶点。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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