Augmentation of neural stem cell proliferation and enhanced differentiation toward neural and oligodendroglia lineages through sonic hedgehog pathway: Cross-activation of Notch1 and SOX10

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-02-06 DOI:10.1016/j.neulet.2024.138098

Arsalan Azizi , Nahid Azarmehr , Maryam Hashemi Shahraki , Roya Aryanpour , Elham Enanat , Parisa Danaee fard , Mehrzad Jafari Barmak , Amir Ghanbari

引用次数: 0

Abstract

The study aimed to understand the impact of the sonic-hedge signal pathway (SHH) on mouse neural stem cells. We manipulated the pathway using purmorphamine (Pur) and Gant 61 and observed the effects on cell viability, neurosphere formation, and gene expression. We found that activating the SHH pathway with Pur increased cell viability, neurosphere formation, and the expression of specific genes, promoting the differentiation of neural stem cells into mature cells. Conversely, inhibiting the SHH pathway with Gant61 decreased cell viability and neurosphere formation and suppressed differentiation. This suggests that the SHH pathway plays a crucial role in determining the fate of neural stem cells.

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通过sonic hedgehog通路增强神经干细胞增殖并向神经和少突胶质细胞谱系分化：Notch1和SOX10的交叉激活

这项研究旨在了解声音对冲信号通路（SHH）对小鼠神经干细胞的影响。我们使用purmorphamine （Pur）和gant61操纵该通路，观察其对细胞活力、神经球形成和基因表达的影响。我们发现，用Pur激活SHH通路可以提高细胞活力、神经球形成和特定基因的表达，促进神经干细胞向成熟细胞的分化。相反，用Gant61抑制SHH通路会降低细胞活力和神经球形成，并抑制分化。这表明SHH通路在决定神经干细胞的命运中起着至关重要的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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