Juanmin Li, Shunsheng Zhao, Lihua Bai, Xiangrong Liu, Li Shang
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引用次数: 0
Abstract
As an important biological indicator, the abnormity of the lysozyme level is closely related to many diseases. Herein, we devise a novel ratiometric fluorescence aptasensor for lysozyme based on the controllable excimer formation of a perylene probe, N, N'-bis(6-caproic acid)-3,4:9,10-perylene diimide (PDI) induced by cationic silver nanoparticles (Ag NPs). Binding of lysozyme aptamer with multiple phosphate groups to cationic Ag NPs strongly hinders the formation of excimer, yielding intense monomer fluorescence of PDI probe. With the introduction of lysozyme, the adsorption of aptamer on the surface of Ag NPs will be weakened owing to the specific interactions between aptamer and lysozyme, which greatly facilitates the excimer formation of PDI. Based on the monomer-excimer transition triggered by lysozyme, ratiometric fluorescence aptasensor for lysozyme can be established. A good linear relationship between the ratio of monomer intensity to excimer intensity and the logarithm of lysozyme concentration was obtained in the range of 0.25-15 nM, and as few as 0.25 nM lysozyme could be easily detected. Moreover, excellent selectivity for lysozyme detection and satisfactory results in real sample analysis were also achieved. This work provides an innovative platform for the construction of simple, label-free ratiometric fluorescence sensors towards a wide range of analytes based on perylene probe.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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