Dietary Astragalus polysaccharides enhance potency of inactivated Pseudomonas plecoglossicida vaccine in large yellow croaker (Larimichthys crocea)

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-02-01 DOI:10.1016/j.fsi.2024.110107

Yueyang Song , Hui Chen , Huimin An , Yongyang Wang , Jianchun Shao , Meijiao Yan , Jingqun Ao , Xinhua Chen , Weini Zhang

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引用次数: 0

Abstract

Dietary Astragalus polysaccharides (APS) get wide application in aquaculture due to their excellent immunoregulatory effects. However, little is known about the effects of dietary APS on vaccine potency in fish. In the present study, large yellow croakers (Larimichthys crocea) were injected with formalin-inactivated Pseudomonas plecoglossicida after APS feeding for 14 d and then challenged by live P. plecoglossicida on 28 d post-vaccination. The results showed that dietary APS combined with inactivated vaccine could improve the survival rate, and alleviate splenic lesions and bacteria load post-challenge, thus exhibiting a better protection in large yellow croaker against P. plecoglossicida infection than inactivated vaccine treatment alone. Fish in APS + P. plecoglossicida vaccine group expressed a better antioxidant status by possessing a relatively higher serum total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity and a significantly lower malondialdehyde (MDA) content than those in vaccine alone group. Serum lysozyme (LZM) and alkaline phosphatase (AKP) activities, and immunoglobulin M (IgM) titers were all improved in fish of APS + P. plecoglossicida vaccine group compared to fish in vaccine group. Furthermore, fish in APS + P. plecoglossicida vaccine group showed a lower down-regulation of pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-6, and a higher up-regulation of anti-inflammatory cytokine IL-10, immunoglobulin (IgM) and T cell immunity-related cytokines, interferon-γ (IFN-γ), IL-4/13A, and IL-4/13B, when compared with those in fish of vaccine group. These results suggested that dietary APS could assist inactivated vaccine to trigger stronger innate and adaptive immune responses against P. plecoglossicida infection. These findings further uncover the immunoregulatory mechanism of dietary APS, and provide valuable information for prevention and control of bacteriosis in fish.

查看原文本刊更多论文

饲料中添加黄芪多糖可提高大黄鱼（Larimichthys crocea）假单胞菌灭活疫苗的效力。

饲料中黄芪多糖具有良好的免疫调节作用，在水产养殖中得到广泛应用。然而，人们对膳食中APS对鱼体内疫苗效力的影响知之甚少。本研究采用黄斑鱼（Larimichthys crocea）在黄斑鱼（Larimichthys crocea）饲养14 d后注射灭活福尔马林的plecoglosida假单胞菌，接种后28 d再用活的plecoglosida攻毒。结果表明，黄芪多糖联合灭活疫苗可提高大黄鱼的存活率，减轻攻毒后脾脏损伤和细菌负荷，对大黄鱼的免疫保护效果优于单用灭活疫苗。鱼在APS+P。与单用疫苗组相比，棘球蚴疫苗组血清总抗氧化能力（T-AOC）和超氧化物歧化酶（SOD）活性较高，丙二醛（MDA）含量显著降低，表现出较好的抗氧化能力。APS+P组鱼血清溶菌酶（LZM）、碱性磷酸酶（AKP）活性和免疫球蛋白M （IgM）滴度均提高。棘球蜂疫苗组与鱼苗组比较。此外，鱼在APS+P。与疫苗组相比，免疫球蛋白疫苗组的促炎细胞因子IL-1β (IL-1β)和IL-6下调较低，抗炎细胞因子IL-10、免疫球蛋白（IgM）和T细胞免疫相关细胞因子、干扰素-γ (IFN-γ)、IL-4/13A和IL-4/13B上调较高。这些结果表明，膳食黄芪多糖可以帮助灭活疫苗触发更强的先天和适应性免疫反应，以抵抗蜂鸟舌虫感染。这些研究结果进一步揭示了膳食中APS的免疫调节机制，为预防和控制鱼类细菌病提供了有价值的信息。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.