Potent antiviral action detected in Tontelea micrantha extracts against Alphavirus chikungunya.

IF 2.4 4区医学 Q3 CHEMISTRY, MEDICINAL

Drug Development and Industrial Pharmacy Pub Date : 2025-02-01 Epub Date: 2025-01-17 DOI:10.1080/03639045.2024.2449130

Ranieli Paiva Lopes, Millena Alves Máximo Vaz, Fernanda Lopes Ferreira, Grasiely Faria de Sousa, Cintia Lopes de Brito Magalhães, Sidney Augusto Vieira-Filho, Jaqueline Maria Siqueira Ferreira, Antônio Helvécio Tótola, Lucienir Pains Duarte, José Carlos de Magalhães

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引用次数: 0

Abstract

Background: Tontelea micrantha, a notable plant species, has garnered interest for its medicinal properties, including anti-inflammatory, antibacterial and antiviral effects. A vaccine for Chikungunia virus is still under evaluation and no specific antiviral drug has been licensed to date.

Objective: The work investigated antiviral activity of ethyl acetate (EAEF) and methanolic (EMF) extracts from T. micrantha leaves in mammalian cells exposed to Alphavirus chikungunya (CHIKV).

Methods: The cytotoxicity, antiviral activity, selectivity index, effect on viral gene expression, virus production, and mechanisms of action were evaluated.

Results: EAEF and EMF extracts showed anti-CHIKV effects at non-cytotoxic concentrations, with CC₅₀ above 300 μg/mL, EC₅₀ of 18 and 43 μg/mL respectively, and selectivity Index above 4. These concentrations drastically reduce viral yields and CHIKV gene expression and have shown activity both directly on viral particles and at different stages of the viral cycle.

Conclusion: EAEF and EMF showed robust antiviral activity against CHIKV, making them promising candidates for the development of anti-CHIKV drugs.

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薇甘菊提取物对基孔肯雅甲病毒的抗病毒作用。

背景：薇甘菊（Tontelea薇甘菊）是一种著名的植物，因其具有抗炎、抗菌和抗病毒作用而备受关注。基孔尼亚病毒的疫苗仍在评估中，迄今为止还没有特定的抗病毒药物获得许可。目的：研究薇甘菊叶乙酸乙酯（EAEF）和甲醇（EMF）提取物对暴露于基孔肯雅甲病毒（CHIKV）的哺乳动物细胞的抗病毒作用。该植物具有显著的抗炎、抗病毒和抗菌作用。方法：对其细胞毒性、抗病毒活性、选择性指数、对病毒基因表达、病毒产生的影响及作用机制进行评价。结果：EAEF和EMF提取物在无细胞毒浓度下具有抗chikv作用，CC50 > 300μg/mL， EC50分别为18和43 μg/mL，选择性指数> 4。这些浓度大大降低了病毒产量和CHIKV基因表达，并直接在病毒颗粒上和在病毒周期的不同阶段显示出活性。结论：EAEF和EMF对CHIKV病毒具有较强的抗病毒活性，是开发抗CHIKV病毒药物的理想选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Development and Industrial Pharmacy 医学-药学

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.