Comparison of a Commercial SARS-CoV-2 Rapid Antigen Test with Real-Time PCR.

Infectious diseases & clinical microbiology Pub Date : 2024-12-19 eCollection Date: 2024-12-01 DOI:10.36519/idcm.2024.421

Muhammet Çağrı Yıldız, Harun Ağca, Anıl Ardıç, İmran Sağlık, Beyza Ener

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Abstract

Objective: The study compared the mö-screen Corona Antigen Test (Qiagen, Germany) with RT-PCR in suspected COVID-19 patients.

Materials and methods: Two hundred combined oro-nasopharyngeal swabs were collected from patients with suspected COVID-19 to evaluate the analytical performance of the mö-screen Corona Antigen Test compared to qualitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) in symptomatic patients.

Results: The mö-screen Corona Antigen Test showed an overall agreement with a sensitivity of 100%, a specificity of 100%, a positive predictive value (PPV) of 100%, and a negative predictive value (NPV) of 100%. We semi-quantitatively evaluated the mö-screen Corona Antigen Test and found a -0.706 correlation between cycle threshold (Ct) values and antigen test results (p<0.001).

Conclusion: The accuracy achievable by the mö-screen Corona Antigen Test, combined with the rapid turnaround time compared to RT-PCR, suggests that rapid antigen tests can be used for rapid diagnosis.

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商用SARS-CoV-2快速抗原检测与实时荧光定量PCR的比较

目的：比较德国Qiagen公司mö-screen冠状病毒抗原检测与RT-PCR对疑似COVID-19患者的检测结果。材料与方法：收集疑似COVID-19患者的200份口鼻咽联合拭子，比较mö-screen冠状病毒抗原检测与定性实时逆转录酶聚合酶链反应（RT-PCR）在有症状患者中的分析效果。结果：mö-screen冠状抗原检测结果总体一致，敏感性100%，特异性100%，阳性预测值（PPV） 100%，阴性预测值（NPV） 100%。我们对mö-screen冠状病毒抗原检测方法进行了半定量评估，发现周期阈值（Ct）值与抗原检测结果的相关性为-0.706 (pp结论：mö-screen冠状病毒抗原检测方法具有较高的准确性，且与RT-PCR相比周转时间短，提示快速抗原检测方法可用于快速诊断。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Infectious diseases & clinical microbiology

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