Diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis.

IF 0.5 Q4 CLINICAL NEUROLOGY
Zoofa Zayani, Etrat Hooshmandi, Afshin Borhani-Haghighi, Mousa Rahimi, Vahid Reza Ostovan, Nima Fadakar, Reza Tabrizi, Mahnaz Bayat, Seyedeh Shamim Hojati, Negin Gharbi, Hamideh Mahmoudinasab
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引用次数: 0

Abstract

Background: Long non-coding ribonucleic acids (lncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of antisense non-coding ribonucleic acid in the INK4 locus (ANRIL) and myocardial infarction associated transcript (MIAT) in patients with cerebral venous thrombosis (CVT). Methods: In this study, fifty patients with CVT and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIAT in the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used to investigate the association of lncRNAs with clinical characteristics in patients with CVT. Receiver operating characteristic (ROC) curve analyses were conducted to evaluate the diagnostic value of two lncRNAs in patient assessment. Results: The relative expression of lncRNAs ANRIL and MIAT significantly decreased in patients compared to the control. ANRIL and MIAT were shown as potential markers for discriminating patients with CVT from the healthy controls with an area under the curve (AUC) of 0.98 and 0.99, respectively. Conclusion: For the first time, we found down-regulation and diagnostic potential of lncRNAs-ANRIL and MIAT in the blood of patients with CVT.

脑静脉血栓患者血液中lncrna - anril和MIAT的诊断潜力。
背景:长链非编码核糖核酸(lncRNAs)被认为可能是循环卒中的指标。本研究关注脑静脉血栓形成(CVT)患者INK4位点(ANRIL)和心肌梗死相关转录本(MIAT)反义非编码核糖核酸的表达状况。方法:本研究纳入50例CVT患者和100例年龄/性别匹配的个体作为对照。采用实时荧光定量聚合酶链反应(RT-PCR)法测定入院后24小时循环ANRIL和MIAT水平。我们使用独立的双样本t检验比较了患者和对照组之间ANRIL和MIAT的表达水平。采用亚组分析探讨lncrna与CVT患者临床特征的关系。采用受试者工作特征(ROC)曲线分析,评价两种lncrna在患者评估中的诊断价值。结果:与对照组相比,患者中lncRNAs ANRIL和MIAT的相对表达量显著降低。ANRIL和MIAT是区分CVT患者和健康对照的潜在标志物,曲线下面积(AUC)分别为0.98和0.99。结论:首次发现CVT患者血液中lncrna - anril和MIAT的下调及诊断潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Journal of Neurology
Current Journal of Neurology CLINICAL NEUROLOGY-
CiteScore
0.80
自引率
14.30%
发文量
30
审稿时长
12 weeks
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