Msx1-Modified Rat Bone Marrow Mesenchymal Stem Cell Therapy for Rotator Cuff Repair: A Comprehensive Analysis of Tendon-Bone Healing and Cellular Mechanisms.

IF 2.1 3区医学 Q2 ORTHOPEDICS

Journal of Orthopaedic Research® Pub Date : 2024-12-31 DOI:10.1002/jor.26039

Kang Liu, Xia-Wei Fu, Zi-Min Wang

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Abstract

This study investigates the therapeutic potential of Msx1-overexpressing bone marrow mesenchymal stem cells (BMSCs) in enhancing tendon-bone healing in rotator cuff injuries. BMSCs were genetically modified to overexpress Msx1 and were evaluated in vitro for their proliferation, migration, and differentiation potential. Results demonstrated that Msx1 overexpression significantly increased BMSC proliferation and migration while inhibiting osteogenic and chondrogenic differentiation. In a rat model of acute rotator cuff injury, Msx1-BMSCs embedded in a hydrogel scaffold were implanted at the tendon-bone junction. Micro-CT analysis revealed substantial new bone formation in the Msx1-BMSC group, and histological evaluation showed organized collagen and cartilage structures at the repair site. Biomechanical testing further confirmed enhanced structural strength in the Msx1-BMSC-treated group. These findings suggest that Msx1 modification enhances BMSC-mediated repair by promoting cell proliferation and migration, facilitating tendon-bone integration. This Msx1-based approach presents a promising strategy for advancing regenerative therapies for rotator cuff injuries.

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用于肩袖修复的 Msx1 改性大鼠骨髓间充质干细胞疗法：腱-骨愈合和细胞机制的综合分析。

本研究探讨了过表达msx1的骨髓间充质干细胞（BMSCs）在促进肩袖损伤肌腱-骨愈合中的治疗潜力。对骨髓间充质干细胞进行基因修饰，使其过表达Msx1，并在体外评估其增殖、迁移和分化潜力。结果表明，Msx1过表达显著增加BMSC的增殖和迁移，同时抑制成骨和软骨分化。在大鼠急性肩袖损伤模型中，将水凝胶支架内包埋的Msx1-BMSCs植入肌腱-骨连接处。显微ct分析显示Msx1-BMSC组有大量新骨形成，组织学评估显示修复部位有组织的胶原和软骨结构。生物力学测试进一步证实了msx1 - bmsc处理组的结构强度增强。这些发现表明，Msx1修饰通过促进细胞增殖和迁移，促进肌腱-骨整合，从而增强骨髓间充质干细胞介导的修复。这种基于msx1的方法为推进肩袖损伤的再生治疗提供了一种有前途的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Orthopaedic Research® 医学-整形外科

CiteScore

6.10

自引率

3.60%

发文量

261

审稿时长

3-6 weeks

期刊介绍： The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.