Immunodominant Molecules for the Immunodiagnosis of Strongyloides stercoralis Infection

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-12-12 DOI:10.1016/j.diagmicrobio.2024.116649

Cíntia de Lima Oliveira , Joelma Nascimento de Souza , Nilo Manoel Pereira Vieira Barreto , Weslei Almeida Costa Araújo , Joana R. Sousa , Victor Amazonas Pereira Maraux , Carina da Silva Pinheiro , Maria Gabriela Almeida , Márcia Cristina Aquino Teixeira , Neci Matos Soares

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Abstract

This study aimed to identify immunodominant molecules with potential for S. stercoralis infection immunodiagnosis. Serum samples were tested by an IgG-ELISA using Strongyloides venezuelensis soluble (AgS) and membrane (AgM) antigens, and its protein fractions (<100 and >100 kDa). Proteins were tested by one- and two-dimensional (2D) gel immunoblotting. The ELISA results showed sensitivities, ranging from 82.3 to 85.3 %, while specificities ranged from 60.0 to 93.3 %. Cross-reactivities were observed with sera from individuals infected with other helminths. Immunoreactive protein bands were revealed by Western blotting using both antigens, and a band with molecular weight around 140 kDa of AgS showed 100 % sensitivity and specificity, without cross-reactivity. The 2D immunoblotting revealed 7 spots in the 140 kDa protein with the S. stercoralis pool of serums. The pre-selection of Strongyloides immunodominant proteins that are highly specific and free from cross-reactions is essential for the production of recombinant antigens for the immunodiagnosis of strongyloidiasis.

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免疫优势分子在粪类圆线虫感染免疫诊断中的应用。

本研究旨在鉴定具有粪球菌感染免疫诊断潜力的免疫优势分子。采用IgG-ELISA法检测委内瑞拉圆线虫可溶性抗原（AgS）和膜抗原（AgM）及其蛋白组分（100 kDa）。蛋白质通过一维和二维（2D）凝胶免疫印迹检测。ELISA检测结果敏感性为82.3% ~ 85.3%，特异性为60.0 ~ 93.3%。与其他寄生虫感染个体的血清观察到交叉反应。Western blotting检测两种抗原的免疫反应蛋白条带，分子量约为140 kDa的条带具有100%的敏感性和特异性，无交叉反应性。二维免疫印迹显示，血清粪孢菌池140 kDa蛋白中有7个斑点。预先选择具有高度特异性和无交叉反应的类圆线虫免疫优势蛋白对于生产用于类圆线虫病免疫诊断的重组抗原至关重要。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.