Four SNPs in the untranslated region of the NOTCH2 gene involved in reproductive traits in fertile and anestrum Holstein Friesian cattle.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2024-12-28 DOI:10.1186/s12917-024-04393-1

Karima Gh M Mahmoud, Ahmed S A Sosa, Hassan R H Darwish, Sally Ibrahim, Mahmoud H Hasanain, Essam A Almadaly, Abdelaziz M Sakr, A Sh E Shams, Daniela Elena Ilie

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引用次数: 0

Abstract

Background: There are no studies belong NOTCH2 gene polymorphism in relation to reproductive and productive traits in Holstein cattle. The objective of the present study was to investigate the effect of NOTCH2 gene polymorphisms on productive and reproductive performance of fertile and anestrum cattle.

Methods: The cattle were classified into anestrus for 3-12 months postpartum (n = 115, 37.7%) and normal cyclic fertile (n = 190, 62.3%). Age at 1st calving, calving interval, calf birth weight, milk yield and days of milking data were obtained from the farm records for all animals. Blood samples were collected from fertile and anestrum animals for DNA extraction and the PCR as well as single-strand conformation polymorphism techniques (SSCP) were performed for detection of the NOTCH2 gene polymorphism.

Results: The sequencing results showed that NOTCH2 gene in cattle is polymorphic with 5 genotypes AA, AG, GG, CC and CT. Four novel SNPs as transition single base substitution mutations were detected at 3: 23,192,033 (A/G), 3:23192149 (C/T), 3:23192071 (C/T) and 3:23192143 (G/A). GG genotype of NOTCH2 gene was associated with shorter calving interval and heavier calf birth weight in both fertile and anestrum cattle.

Conclusions: NOTCH2 gene in cattle could be used as a genetic marker involved in reproductive traits in both fertile and anestrum cattle.

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NOTCH2基因非翻译区域的4个snp与可育和未发情荷斯坦弗里西亚牛的生殖性状有关。

背景：目前还没有关于荷斯坦牛NOTCH2基因多态性与繁殖和生产性状关系的研究。本研究旨在探讨NOTCH2基因多态性对可育牛和无乳牛生产和繁殖性能的影响。方法：将牛分为产后3 ~ 12个月无月经（n = 115，占37.7%）和正常周期可育（n = 190，占62.3%）。所有动物的第一次产犊年龄、产犊间隔、犊牛初生重、产奶量和挤奶天数数据均来自农场记录。采集可育动物和无孕动物的血样进行DNA提取，采用PCR和单链构象多态性技术（SSCP）检测NOTCH2基因多态性。结果：测序结果显示，牛NOTCH2基因具有5个基因型，分别为AA、AG、GG、CC和CT。在3:23,192,033 (A/G), 3:23192149 (C/T), 3:23192071 （C/T）和3:23192143 （G/A）位点检测到4个新的过渡单碱基取代突变。NOTCH2基因的GG基因型与可育牛和无乳牛的产犊间隔缩短和犊牛初生体重增加有关。结论：牛NOTCH2基因可作为参与可育牛和无乳牛生殖性状的遗传标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.